Rat Blood-testis Barrier In The Hbv Dna Transfer Dye Protection And Its Influencing Factors

Background & Objective Vertical transmission of HBV is a new and unproved transmission which needs germocyte as carrier to import HBV DNA into embryo,and causes off-spring to become a carrier or a patient.The key point of HBV transmission between father and infant is to illuminate how can HBV transfect sperm cell through blood-testis barrier(BTB).In this study,pBR322-HBV DNA plasmid transfected into rat testicles in different groups,to explore whether BTB can efficiently prevent the plasmid transfecting seminiferous epithelium cells on contorted seminiferous tubules,and approach the factors that effect the integrality of BTB.Methods Twenty rats of Wistar mature male were divided into two groups randomly:experimental group(A)and control group(B),the expression of HBV DNA and the trasfection in contorted seminiferous tubules were measured by polymerase chain reaction(PCR), real-time quantitative PCR(real-time PCR),in situ hybridization(ISH) and transmission electron microscopy(TEM).Resultsâ‘ The existence of HBV DNA can be found in testicle by the detection of PCR In group A;â‘¡The average content of HBV DNA in group A is much higher than group B by the detection of real-time PCR,the two groups have statistical significance;â‘¢In testicle of group A, HBV DNA can be widely found during the outer compartment of seminiferous epithelium and the inner compartment of spermatogenic cells;â‘£Thickened,wrinkled or broken basement membrane and under basement membrane were in the seminiferous tubules,more cytoplasm and vacuolisation were in the sperm of the convoluted seminiferous tubules and the sustentacular cells under electronmicroscope.Conclusionâ‘ Basement membrane in seminiferous tubule of normal rat is integrated, smooth and uniform,but hydrocortisone can cause the abnormality of ultramicrostructure,such as basement membrane,tight junction,supporting cell,and so on;â‘¡Destruction of BTB can increase the risk of HBV DNA infection in testicle;â‘¢Structural abnormalities of seminiferous tubule or destruction of BTB can induce HBV DNA transfection in the whole seminiferous epithelium of rat testicle,and cause higher transfection efficiency.The integrality of BTB plays a main role in protection,unintegrality can make whole spermatogenic cells be easily infected with HBV.