Non-alcoholic Fatty Liver Rat L-fabp And Of Ppar-¦Á In The Dynamic Expression And Significance

Objective: To construct NAFLD model and detect the change of L-FABP, PPAR-αdynamic expression and support rationale for NAFLD clinical diagnosis, therapy and prognosis.Methods: 84 male Wistar rats of (180±10g) weight were divided into two groups randomly with 42 in each group: control group were fed with normal diet; Model group were fed with high-rich diet (normal diet addition of 0.1 lard oil, 0.02 cholesterol and 0.002 cholate in mass fraction ) to establish NAFLD models. The model group were fed with normal diet from the 12th to 18th week. Then the two groups were sacrificed at the 0, 2nd, 4th, 8th, 12th, 16th, 18th weeks respectively. The blood from hearts were used for detecting ALT, TG, CHOL, HDL-C and LDL-C. One part of the liver were dyed with HE and the other were used to detect the change of L-FABP and PPAR-αmRNA by real-time fluorescence quantitative RT-PCR and L-FABP by Western-blot.Results: L-FABP and PPAR-αexpression of normal diet rats did not change obviously. L-FABP mRNA expression of high-rich diet rats increased more obviously (P<0.05) at the 4th week, most obviously (P<0.01) at the 8th and 12th week and more obviously (P<0.05) after 6 weeks of feeding normal diet, but decreased most obviously compared to the 12th week. L-FABP of high-rich diet increased most obviously (P<0.01) from the 2ed to 12th week. PPAR-αmRNA of high-rich diet rats decreaed more obviously (P<0.05) at the 4th week, most obviously at the 8th and 12th week, and more obviously (P<0.05) after 6 weeks of feeding normal diet, but increased most obviously (P<0.01) compared to the 12th week. Steatosis was found at the 4th week, and became the worst but no inflammation was seen at the 12th week, and steatosis improved great after 6 weeks of normal diet.Conclusion: Steatosis model was contructed successfuly and large vesicular steatosis was found at the 12th week, but no Inflammatory response. Appearance of L-FABP expression threshold and down regulated expression of PPAR-αcould play a very important role in steatosis development. Simple steatosis would improve great through normal diet.