Cerebral Ischemia And Reperfusion In Brain Tissue Of Cd40l, Mmp-3 With Brain Injury And Drug Intervention

PartⅠThe expression of CD40L,MMP-3 in brain of rats with focal cerebral ischemia-reperfusionObjective To study the mechanism of CD40L,MMP-3 involved in the injury induced by cerebral ischemia-reperfusion through observation of the expression of CD40L,MMP-3 in brain of rats with focal cerebral ischemia-reperfusion.Methods The middle cerebral artery (MCA) was occluded with thread to make rats into brain ischemia-reperfusion model. Wistar rats were randomly divided into:normal group(N group), sham-operation group(sham group), ischemia-reperfusion group(IR group) 6h,24h,48h,72h,7d. Their expressions of CD40L,MMP-3 in ischemia brain tissues were measured by immunohisto chemi-cal methods. The water content was calculated by dry and wet weight method. The neurological function deficit score was graded by classical Zea longa methods.Results The expressions of CD40L,MMP-3 (P<0.05),neurologicol scores (P<0.01) and subtract brainedema (P<0.05)in IR groups were higher than those in N and sham group(P<0.01). The CD40Land MMP-3 had already significantly expressed on corresponding positive cells at 6h reperfusion, The water content began to increased at 6 hours, then them increased at 1 d and reached peak level at 2d, after this, the level of CD40L,MMP-3 and the water content began to decrease up to 7d.Conclusion The CD40L,MMP-3 expressed in brain of rats with focal cerebral ischemia-reperfusion are involved in the injury induced by cerebral ischemia-reperfusion. MMP-3 may play an important role in vascular derived brain edema by degradation of extracell matrix and Blood-uiibrain barrier.PartⅡInhibitive action of atorvastatin on CD40L,MMP-3 in ischemic brain tissue after reperfusion in ratsObjective To study the neuroprotective mechanism of atorvastatin against the injury induced by cerebral ischemia-reperfusion by observation of the expression of CD40L,MMP-3 in brain of rats with focal cerebral ischemia-reperfusion in Atorvastatin treatment group.Methods The middle cerebral artery (MCA) was occluded with thread to make rats into cerebral ischemia-reperfusion models. Wistar rats were randomly divided into:sham-operation group (sham group), ischemia-reperfusion group (IR group), atorvastatin treatment group (AT group). Rats were intragastric injected with administration of 10ml/kg of 2.5mg/ml atorvastatin in atorvastatin-treated group for 7days before the model of middle; The sham group was pretreated with normal saline for 7days before the model of middle cerebral artery occlusion (MCAO) was establishe-d.Their expressions of CD40L,MMP-3 in brain tissues were measured by immunohistochemical methods. The water content was calculated by dry and wet weight method. The neurological function deficit score was graded by classical Zea longa methods.Results The expressions of CD40L(P<0.01),MMP-3 (P<0.05), neurologicol scores (P<0.01). subtract brainedema (P<0.01) in AT groups were lower than those in IR group(P<0.01), significantly higher than those in sham groups (P<0.01).Conclusion Atorvastatin can lighten the level of vasogenic brain edema and improve the neurological deficit scores, Atorvastatin may protect neural function by downregulating CD40L,MMP-3 expressions during focal cerebral ischemia-reperfusion.