Intragastric Ph Intelligence To Ammonia Microemulsion Gel Experimental Study Of Prevention And Treatment Of Rats With Hepatic Encephalopathy

MBGs-RA respectively, blank control group was administrated with equivalent blank microemulsion-based gels, lactulose control group was administrated with equal volume lactulose, normal control group and HE group were administrated with normal saline, and acetic acid control group was administrated with 23.1 percent acetic acid solution accordingly. Administration was repeated once a day,4 times in total.5. The electroencephalogram(EEG) of all rats were determined to observe the effects of MBGs-RA on the EEG of rats with HE at the fourth day of experiments using biological function experimental system.6. Blood was drawn from angular vein to test liver function using alanine aminotransferase and total bilirubin reagent kit, and blood was drawn from inferior vena cava to detect blood ammonia using automatic dry-type biochemical analysator after determing the EEG. Colon was weighed and dealt with by normal saline to detect intestinal tract ammonia using automatic dry-type biochemical analysator before executing all the rats.7. Pathological sections were prepared for group comparison.ResultBehavioral comparison among the rat groupsCompared with normal control group, rats behavior in blank control group was not significantly different (P>0.05), and it in HE group appeared depressed, reduced activities, unresponsive and drowsiness. Besides that, hours of sleep was prolonged(P<0.05), food-intake, weight loss and grade score of HE were increased (P<0.05). Compared with HE group, indexes above in high, medium and low dose of MBGs-RA groups, acetic acid control group and lactulose control group were improved to varying degrees, hours of sleep, food-intake, weight loss and grade score of HE were restored (P<0.05), the effects on high and medium dose of MBGs-RA groups were better than other treatment groups (P<0.05). Hours of sleep, weight changes as well as grade score of HE were negatively correlated with the doses (P<0.05), except food-intake (P>0.05).Comparison of EEG among the rat groupsEEG in normal control group and blank control group was normal which appeared a waves mainly, with frequencies of 8-13 times per second, and voltages of 50～100μV. EEG in HE group showed significantly different, which appeared extensive wide 5 waves, with frequencies of 5～7 times per second, and voltages of 160～280μV. EEG in high and medium dose of MBGs-RA groups showed mild to moderate abnormality, which appeared slow a waves, mingled manyθwaves, with frequencies of 8～11 times per second, and voltages of 150～250μV. EEG in the low dose of MBGs-RA group, acetic acid control group and lactulose control group showed moderate abnormality, which appeared 0 waves mainly, with frequencies of 7-9 times per second, and voltages of 130～200μV.Comparison of blood ammonia and intestinal tract ammonia among the rat groupsCompared with normal control group, blood ammonia and intestinal tract ammonia in blank control group were not apparently distinct (P>0.05), which in HE group and each treatment group were increased entirely (P<0.05). Compared with HE group, indexes above in all the treatment groups were decreased (P<0.05), and the effects of high and medium dose of MBGs-RA groups were better than low dose of MBGs-RA group, acetic acid control group and lactulose control group (P<0.05). There was no significant difference among low dose of MBGs-RA group, acetic acid control group and lactulose control group (P>0.05). Negative correlation between doses and indexes above was revealed (P<0.05).Comparison of biochemical liver function (ALT, TBIL) and liver pathology among the rat groupsCompared with normal control group, ALT, TBIL and liver pathology in blank control group were not significantly different (P>0.05), which in HE group and each treatment group were different entirely(P<0.05); Compared with HE group, indexes above in all the treatment groups were different (P<0.05), except TBIL in acetic acid control group (P>0.05), and the effects of high and medium dose of MBGs-RA groups were better than low dose of MBGs-RA group, acetic acid control group and lactulose control group (P<0.05). There was no significant difference among low dose of MBGs-RA group, acetic acid control group and lactulose control group (P>0.05). Negative correlation between doses and ALT, TBIL was revealed (P<0.05). Conclusion1. pH-sensitive MBGs-RA could decrease blood ammonia and intestinal tract ammonia and improve liver function, behavior and electroencephalogram of rats.2. Effects of high and medium dose of pH-sensitive MBGs-RA groups on the prevention and cure of hepatic encephalopathy were evident, and were better than lactulose and acetic acid control groups.