Microdialysis Technique Used In Rhynchophylline Pharmacokinetic Study

Purpose: To investigate the influence of different factors (including the velocity, concentration,sampling interval) on in vivo and in vitro recovery of Rhy by micro-dialysis, explore the feasibility of using microdialysis technique in pharmacokinetic studies and establish a general method..Method: Analysis method using HPLC analysis of samples; Determination of probe recovery by incremental, reduction method in vitro. And the in vivo anti-dialysis recovery be determined; Using DAS2.0fitting procedures by compartment model, calculating pharmacokinetic parameters.Result: The variation of recovery rate measured by incremental and reduction method under the same flow rate exerted similar trends, which provided sound basis for in vivo experiment. In vivo recovery rate showed no relevance to the concentration. The recovery rate demonstrated preferable stability. The optimal experimental conditions of in vitro recovery were determined as follows: velocity as 2.0μl/min, sampling interval as 20min and sample volume collected as 40μl. See from the concentration-time curve that Rhynchophylline in vivo after intravenous injection of processes for the two-compartment model, and the elimination and distribution is a dynamic process.Conclusion: The in vitro and in vivo pharmacokinetic study of rhynchophylline by micro dialysis in this experiment exhibit high specificity and precision. Blood rhynchophylline concentration complies with linear dynamics and eliminates fast with a wide range of distribution. Plasma concentration data suggest that in vivo after intravenous rhychophylline process for the two-compartment model , the elimination and distribution is a dynamic process.