Fiber Grams Particle Expression In Bleomycin-induced Pulmonary Fibrosis In Rat Tnf-¦Á Cytokines

Purpose: To observe XianKe Granule on pulmonary fibrosis in rats at different times of the effects of TNF-αand in order to confirm preventment and treatment of XianKe Granule on pulmonary fibrosis.Methods: In this study, bleomycin-induced pulmonary fibrosis in rats. Wistar rats of clean grade the filter 108, using random number table is divided into six groups, normal control group, model group, prednisone group, XianKe Granule low-dose group, middle-dose group and high-dose group, 18 rats in each group. Ordinary special feed were fed. Beginning the day after modeling the normal control group, model group, the standard weight according to 10ml/kg fed with normal saline, prednisolone group by 5g/kg weight, XianKe Granule of high, middle and low-dose groups respectively 10g/kg, 5g/kg, 2.5g/kg weight of XianKe Granule aqueous solution. Continuous administration, respectively, the 7th, 14th and 28th day, the abdominal aorta blood, using ELISA in serum were determined during the TNF-αlevels.Results: Rats'TNF-αexpression of various periods in model group were higher than other groups differ significantly(p<0.01), which reached the peak on the 7th day, then decreased gradually, but still were higher than others'; the expressions of prednisone group and XianKe Granule high, middle and low-dose group were higher than the normal group, lower than the model group.On the 7th day the expressions of TNF-αin order from high to low were XianKe Granule low-dose group, prednisone group, high-dose group and middle-dose group. On the 14th day the order from high to low were XianKe Granule low-dose group, high-dose group, prednisone group and middle-dose group.The order of the 28th day is the same as the 7th's order. In various periods, prednisone group compared with the XianKe Granule middle-dose group were significantly different (p<0.05), and the other XianKe Granule group were not significant difference (p>0.05); high-and low-dose group and the middle dose group were significantly different (p<0.05 or p<0.01).Conclusion: XianKe Granule could inhibit weight declining, compared with the model group were significantly difference (p<0.05 or p<0.01). XianKe Granule middle-dose can significantly reduce the different periods of pulmonary fibrosis in rat TNF-αcontent, compared with the prednisone group were the obvious difference (p<0.05), and that shows XianKe Granule to pulmonary fibrosis have more obviously preventive and therapeutic effects, its mechanism may be related to inhibiting the expression of TNF-α.