| Caramel pigment is a kind of synthetic edible pigment, which is widely used as a kind of food additives. At present people gradually get attention to the safety of the caramel pigment and the concentration of 5-HMF must be restricted. In this paper, caramel pigments (ECP) without 5-HMF was prepared by erythrose-ammonium sulfite model Maillard reaction under the optimum process conditions, fractionated by ultra-filtration. A intermediates of the Maillard reaction products was isolated and identified. Moreover, Anti-tumor and antioxidant activity of ECP was researched on the base of the structure of melanoidinen in order to provide scientific basis for applications in food industry.Firstly, the highest color intensity is 18054.43EBC under the optimum process conditions that is 140℃for heating temperature,40min for heating time,459g/L of erythrose and 4% of (NH4)2SO3 (by NH4+),determined by the method of twice revolve combination design.The ECP fractionated by ultra-filtration are homogenization and uniform. ECP above 100kDa level can reach 95526.32EBC of color intensity,3.59 of red index and 5.51 of yellow index, which is suitable for market.Referenced to GB8817-2001, the ECP is negatively charged, above 25h of resin change time in 30%-50%, contains no 5-HMF and is suitable for soft drink.An intermediates of the Maillard reaction products was isolated and identified by Mass spectrum, which certified that there is no 5-HMF yielded in Maillard reaction. The structure of the intermediate is induced and the molecular formula is C8H10O3.Lastly, The structure of melanoidinen was discussed by the method of elementary analysis, IR and pyrolysis, based on which Anti-tumor and antioxidant activity of ECP was researched. The evidences showed that the pyrolysate of melanoidinen contained SO2, chain hydrocarbon and furan ring. MDA-MB-231 cells incubated in 24h and 72h under diverse ECP volumes were inhibited under ECP 10k-50kDa and were induced under ECP50k-100kDa and ECP 100kDa-0.22μm. |
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