DNA-,RNA-binding Behaviors And Cytotoxic Properties Of Ru(â…¡) And Co(â…¢) Complexes

DNA and RNA are both the genetic material in organism, which play an important role in many sorts of biological processes, meanwhile, they are attractive targets for potential therapeutics. Ruthenium(â…¡) polypyridyl complexes have rich photochemical and photophysical properties, and so forth. Because of these factors, during the past decade the transition metal complexes binding to nucleic has aroused people a great interests to study and chose the novel transition metal polypyridyl complexes which can be potential applied in nucleic-structure probes, nucleic-molecular light switches, anti-cancer drugs and so on. Co(â…¢) polypyridyl complex is also one sorts of the most attractive transition metal complexes other than the ruthenium(â…¡), the Co(â…¢) complex has their own characteristics which are different from the Ru(II) complexes. Herein, we synthesized two Ru(â…¡) complexes and two Co(â…¢) complexes. We hope that our studies are helpful to the development of DNA and RNA molecular probes and new therapeutic regents. This thesis consists of the following parts:Fitstly, the thesis introduced the basic information about nucleic the three non-covalent modes between the complex and nucleic such as electrostatic, intercalative andgroove binding, and the new vista of research for bioinorganic Chemistry, and so forth.In chapter 2, we synthesized two Ru(â…¡) complexes [Ru(IP)2DPBPD(NH2)2]2+1 and [Ru(IP)2BPIP]2+2, and we characterized the complexes by elemental analysis,'H NMR and ES-MS. The binding behaviors between the complexes and nucleic were studied by different spectrophotometric methods and viscosity measurements. Through the experiment we reached these conclusions:the binding mode of the complex 1 to CT-DNA and yeast tRNA are intercalation, however, the complex 2 binds to CT-DNA and yeast tRNA by a partial intercalative mode, and both of the complexes with RNA binding are much stronger than DNA binding.In chapter 3 and 4, we synthesized two Co(â…¢) complexes [Co(phen)2BPIP]3+3 and [Co(phen)2PPIP]3+4, and we characterized the complexes by elemental analysis, 1H NMR. The binding behaviors between the complexes and nucleic were studied by different spectrophotometric methods and viscosity measurements. Through the experiment we reached these conclusions:the binding mode of the complex 3 to CT-DNA and yeast tRNA are intercalation, however, the complex 4 binds to CT-DNA and yeast tRNA by a partial intercalative mode, both of the complexes with RNA binding are much stronger than DNA binding.Moreover, to study these complexes'tumor-fighting properties we used anti-cancer test in vitro by the MTT method(MTT=(3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide). The results indicated the complexes could display different activities against some kinds of tumor cell HL-60 (cervical), HepG2 (hepatocellular), through these experiments, we hope that we can search the efficient anti-caner medicines as quickly as possible.