| The definition and classification of plant seed-borne diseases were introduced in this paper. At the same time, the progress of detecting plant seed-borne diseases was also discussed. The techniques used in detecting the fungal diseases such as washing microscopic examination, moisture culture, inoculation, PCR method were highlighted. Meanwhile, the techniques used in detecting the viral diseases such as symptom observation (including differential host test), serological methods, molecular biology methods were also described in detail.As cucumber green mottle mosaic virus, tobacco ringspot virus and Cladosporium cucumerinum for the examples, focuses on the detection and research methods of seed-borne virus diseases of plants and plant species of fungi disease, and seed-borne disease detection methods are summarized and prospects.And at the end of the paper, the tendency of development in detecting plant seed-borne diseases and its prospect were also forward promoted.Cucumber green mottle virus is a typical seed-borne virus, in this study, inoculation of cucumber green mottle virus, cucumber seeds as materials. And this study established a variety of methods for the rapid detections of cucumber green mottle virus, including DAS-ELISA method, the general RT-PCR method, the immune capture RT-PCR, fluore scence RT-PCR, RT-PCR and immunomagnetic beads method, and compared the sensitivity of each method. The cucumber seeds infected with cucumber green mottle mosaic virus were used as a test material, and a high sensitivity method of directly detecting the virus was established by immunomagnetic separation and reverse transcriptase-polymerase chain reaction (IMS-RT-PCR). The test results showed that when the concentration of envelope monoclonal antibody of magnetic beads was 1.5×10-5 mg/mL, the lowest detectable antigen concentration was 7.813×10-3 mg/mL, about a quantity of the virus in 8 ng cucumber seeds, indicating the sensibility of this method was 8 times higher than that of the immunocapture RT-PCR (IC-RT-PCR).Tobacco ringspot virus owe to mosaic virus family, and dispersal by seed, The pass design TRSV coat protein specific primers, and using of laboratory toxicity of existing sources as testing TRSV material. This study established many methods for testing TRSV, including common RT-PCR method, the immune capture RT-PCR method, real-time fluorescence RT-PCR (SYBR Green method) of the detection method. And the sensitivity of various detection methods were studied spirits, to determine the different ways the virus can detect the lowest concentration.Cucumber scab fungi are important plant species diseases, in recent years, was one of the important diseases on Cucumber production, and made serious harm to China cucumber and other vegetables and fruit production. In this study, using strains -11279, -38730 strains and strains - Shenyang as the experimental material, and the cultivation of fungi withPDA medium, in the dark and light in both conditions for spore germination at 20℃. The results show that in the dark conditions germination for spores was easy. Then made three strains inoculated in health cucumber leaves, removed cucumber seeds when cucumber was matured, and conducting a series of PCR methods. Using fungal universal primers ITS4 / 5 as preliminary screening, and then as specific primers HGHX-F1 / R and WF / R and the NF / R for ordinary PCR and nested PCR reactions, DNA amplification of specific bands obtained and the DNA sequence. The bacteria form spores and bacteria combine the results of DNA sequence, which identified three species of fungi, this is a cucumber scab pathogen. |
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