Fabrication And Properties Research Of Novel Fluorescent Nanoparticles For Cupric Ion Detection

Copper is a indispensable nutrient substance for human health, it plays a crucial role in hemopoiesis, metabolism, growth, and immune system. However, excess copper intake can lead to protein denaturation and inactivation, generating toxic action to organism. It was shown that the Cu/Zn ratio in vivo had something to do with tumorigenesis . Fish will die at the Cu2+ concentration of l mg/L. Algae and mollusca are extremely sensitive to Cu2+, the drinking safe concentration is under 10μg/L. Metallurgy and electroplating is the main source of Cu2+. Owing to long-term and massive used by people, copper is changing into a main pollutant. It was very necessary to develop a kind of high sensitivity and quick detecting method of copper ions. There aer many detective methods for Cu2+ studied for a long time, such as atomic absorption, emission spectroscopy[1-3], atomic fluorescence spectrometry[4] and electrochemistry method. However, these methods need expensive facility and complex sample preparing process. Different from above methods, the fluorescence technique has become a powerful tool for sensing and imaging trace amounts of metal ions because of its simplicity, sensitivity and not required external power supply. Since 1920s, because of its high distinguishability, short response time and simple preparation, nanotechnology has been broadly used in environmental science, biomedical science and food science.In this paper, one kind of inorganic/organic nanohybrid liposomal was synthesized and some nanoparticle were prepared by this new liposomal. The hydrophobic fluorescence dye Nile Red (NR) was embedded in these nanoparticles. The surface of nanoparticles are covered Si-O-Si gridstructure. It was found that the kind of inorganic/organic nanohybrid liposomal cerasomes are not only retain same characteristic as lipid which was hydrophilic surface, bilayer and hydrophilic inner cavity. On the other hand, it had much higher stability than lipid. The novel inorganic/organic nanohybrid liposomal cerasomes can maintain the whole three-dimensional vesicle structure and can not be destructed under the surfactant, indicating the high stability of nanohybrid liposomal cerasomes prevents the dye leakage. It was impossible for lipid. Furthermore, nanohybrid liposomal cerasomes has uniformity and regular-shaped, about 140 nm and zeta potential was positive. The nanohybrid liposomal cerasomes distribution is clarification and Si-OH groups in nanoparticles surface making them has abundant phy-chemical property. The ultrathin membrane of nanohybrid liposomal cerasomes with nano-structure was prepared by layer-by-layer self-assembly technology.DMPE can be combined with copper ions which are as energy accepter accept the energy from NR, resulting in fluorescence quenching, thus, reslizing copper ions detection in water. Nanopartices prepared by thin-film aqueous method and probe ultrasonic method. The research shown that DMPE ratio in nanohybrid liposomal cerasomes has an effect on the stability of nanoaprticles and Cu2+ sensitivity. Nanoparticles obtained the best stability(intact storage for 10 days at 4℃), and the highest sensitivity(the detective limit is 0.25μM). Moreover, different anions exhibited little influence on the determination of copper ions even could be neglected, thus, nanohybrid liposomal cerasomes are ideal material for fast and high sensitivity detection for copper ions.