Study Of Cyclodextrin-induced Non-deoxygenated Room Temperature Phosphorescence Of Fluorene And Aminofluorene

Chapter 1 The development and application of room temperature phosphorescence (RTP) spectroscopy are briefly introduced. And the non-deoxygenated RTP, especially the cyclodextrin-induced RTP in the presence of oxygen is reviewed in detail.Chapter 2 Strong room temperature phosphorescence of Fluorene is synergistically induced by y-cyclodextrin (γ-CD) and 1,2-dibromoethane (1,2-DBE) without removal of oxygen dissolved in the solution because of the formation of ternary complex of Fluorene,γ-CD, and 1,2-DBE with 1:1:1 stoichiometry. Under optimum experimental conditions, the room temperature phosphorescence intensity was liner with fluorene concentration in the range of 1.8 X 10-7mol/L～2.5×10-6mol/L and 2.5×10-6mol/Ly～2.75×10-5mol/L, respectively. The detection limit was 1.53×10-9 mol/L. The proposed method was applied in the determination of fluorene in synthetic seawater samples with the relative standard deviations of 2.06～2.87%(n=5) and the recoveries in the range of 96.7%～98.3%。Chapter 3 Upon addition of small amount of 1,4-Dibromobutane (1,4-DBB),1-Aminofluorene displays strong room temperature phosphorescence inγ-cyclodextrin(γ-CD) solution without deoxygenation. Various factors influencing the room temperature phosphorescence intensity of 1-Aminofluorene were investigated. A simple, rapid and sensitive method for the determination of 1-Aminofluorene was established.Chapter 4 In the presence of small amount of 1,4-Dibromobutane (1,4-DBB),γ-cyclodextrin (γ-CD) can induce 2-Aminofluorene to emit strong room temperature phosphorescence without deoxygenation. Various factors influencing the room temperature phosphorescence intensity of 2-Aminofluorene were investigated. Under optimum experimental conditions, the room temperature phosphorescence intensity can be correlated linearly with 2-Aminofluorene concentration in the range of 1.0×10-6 mol/L～6.0×10-6 mol/L and 6.0×10-6 mol/L～2.6×10-5 mol/L respectively. The detection limit was 2.7×10-9 mol/L. Therefore the proposed method enables the determination of 2-Aminofluorene.Chapter 5 Room temperature phosphorescence of Fluorene as well as 1-Aminofluorene and 2-Aminofluorene induced by y-cyclodextrin (γ-CD) with 1,4-Dibromobutane (1,4-DBB) as a third component were comparatively investigated. In combination with the experimental results described in Chapter 2～4, three kinds of inclusion modes between y-CD and Fluorenes were proposed based on the Semiempirical quantum mechanical calculations. Meanwhile, the inclusion mode was studied based on 1HNMR technoque.