Fabrication Of Silk Fibroin Nanofibers Loaded With Vitamin A And E By Green Electrospinning And Evaluative Study On Its Biocompatibility

Recently, with the popularization of the idea-"skin care" and the pollution issue, a large number of skin-care nanofibers and textiles are emerging in response to a common need for health. Silk fibroin is one of the biomaterials having excellent biocompatible. And it has recently found applications in the field of biomedical engineering ranging from cartilage, ligament, skin, vascular grafts,etc. Vitamin A and E, two essential vitamins, which are required for normal body functioning, also have excellent skin care function.Electrospinning is a relatively robust and simple way of fabricating ultrafine polymeric fibers with high surface area and excellent mechanical properties. Electrospun fibers have been successfully applied in various fields, such as, filtration, protective clothing, tissue engineering, wound dressing, drug delivery, capacitor, sensor, etc. Recently, this technique also has been applied in the field of cosmetics, acting as a facial mask for the treatment of skin hearing or skin cleaning with or without various additives.Based on the above-mentioned reasons, the objective of this work is to fabricate mats of electrospun silk fibroin nanofibers with the capability of controlled released of vitamin A and vitamin E TPGS. In this study, vitamin A and vitamin E were successfully incorporated into nanofibers by means of mix and emulsion electrospinning. These nanofiber mats were characterized using scanning electron microcopy, Fourier-transform infrared and X-ray diffractometry.The release behaviors from the fibers were examined. After post-treatment, silk fiborin is suggested to be barley degradable over months,so diffusion could be mainly responsible for the drug release.All series of nanofiber mats exhibited a sightly burst release during the first 30 mins, followed by a slow release over 72 hours. Also, the composite nanofibers were investigated for cytotoxicity and oxidative stress, demonstrating the vitamin-loaded SF nanofibrous mats has good biocompatibility, excellent antioxidant potential for reduction in oxidative stress in L929 cells, and a potential application in skin-care or skin tissue regeneration.The results show that electrospun silk fibroin nanofibers containing vitamin A and E which might provide an attractive alternative to the application on skin care, wound dressing and skin tissue engineering.