Study On RIZ1 Gene Promoter Methylation Status In Human Esophageal Squamous Cell Carcinoma Tissue

Objective:To detect the CpG Islands which in promoter region methylation status of RIZ1 gene in the human esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue. And analyze their relationship with the clinical and pathological features of the human esophageal squamous carcinoma.Methods:The human esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue specimens and their clinical and pathological data were collected.Methylation-specific PCR(MSP) was used to investigate the CpG Islands which in promoter region methylation status of RIZ1 gene in the carcinoma tissues, the adjacent tissues and the normal tissues from 47 esophageal squamous cell carcinoma patients. The relationship between the CpG Islands which in promoter region methylation status of RIZ1 gene and the clinical and pathological features of the patients was analyzed combined with the related data such as gender, age, family history, cell differentiation of tumor, TNM classification of tumor and clinical stage of the 47 esophageal squamous cell carcinoma patients.Results:The cases of CpG Islands which in promoter region methylation of RIZ1 gene were found 26,3,0 in the esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue specimens of 47 esophageal squamous cell carcinoma patients respectively. The positivity rate of promoter region methylation of RIZ1 gene was significantly higher in esophageal squamous carcinoma tissue than that in adjacent tissue and the normal tissue (55.3%vs.6.4%and 0.0%, P<0.01), but no significant difference was detected between the adjacent tissue and the normal tissue (P>0.05). We found that the CpG Islands which in promoter region methylation status of RIZ1 gene in the human esophageal squamous carcinoma had no obviously relationship with the data of gender, age, family history, cell differentiation of tumor, TNM classification of tumor and clinical stage (P>0.05, respectively) Conclusion:Promoter hypermethylation of RIZ1 gene is present in the human esophageal squamous carcinoma, suggesting that the promoter hypermethylation of RIZ1 gene was associated with the tumorigenesis of human esophageal squamous carcinoma, it may be a significant contributing factor to tumour development in human esophageal squamous carcinoma. The CpG Islands which in promoter region methylation of RIZ1 gene may be one of the earlist events in the occurrence of human esophageal squamous carcinoma, it may provide help in the early detection of esophageal squamous carcinoma.