The Primary Research Of The Influence Of SOCS1 On The Antiviral Activity Of Interferon-α Against HBV

Objective : To investigate the relationship between SOCS1 and the antiviral activity of IFN-αagainst HBV.Methods: The HepG2.2.15 cells, treated by 1,000IU/ml IFN-αfor 48 h, were divided into three groups, the experimental group (transfected with pcDNA3.1-SOCS1 plasmid),the control group (transfected with pcDNA3.1 plasmid) and blank group (HepG2.2.15 cells without 1,000IU/ml IFN-αtreatment). After transfection, Real-Time PCR and chemiluminescence assay were employed to detecte HBV DNA, HBsAg, HBeAg as well as the expression of 2'-5'OAS, MxA, PKR and SOCS1 proteins. Meanwhile, HepG2 cells were transiently transfected with HBV core protein-expressed plasmid pHBc-EGFP, and the expression of SOCS1 were also analysed by Real-Time PCR assay.Results: The SOCS1 protein could be expressed in HepG2.2.15 cells transfected with pcDNA3.1-SOCS1 (wild-type) plasmid. Further, in our study, treatment with 1,000IU/mL IFN-α, the expression of HBeAg, HBsAg and HBV-DNA, in experimental group, were found significantly increased (P<0.05), compared with the control group. And the level of antiviral proteins like as MxA, PKR, 2'-5'OAS were found significantly decreased. Meantime, the expression of SOCS1 protein was decresed in HepG2 cells transfected with pHBc-EGFP plasmid.Conclusion: In cell lines, the SOCS1 exerts the antagonistic action of IFN-αantiviral activity by down-regulating the expression of antiviral proteins, like as MxA, PKR, 2'-5'OAS. The Hepatitis B Virus Core Protein (HBc ) decreases the expression of SOCS1, and there is different mechanism between the SOCS1 and HBc protein on the antiviral activity of interferon-αaganist HBV.