EZH2 And PTEN Protein Expression In Ovarian Tumors And Clinical Significance

Objective:To investigate the expression of EZH2 and PTEN protein in ovarian tumor tissue, and to explore the relations to clinicopathologic characters of ovarian carcinoma and the relationship of themselvesMethods:Ovarian cancer tissue microarray(OV1005) which was Choosed from chaoying biotechnology company, containing normal oarian tissue 20 cases, benign ovarian tumor 18 cases, border ovarian tumor 7 cases, ovarian carcinoma 55 cases. Then, to detect the expression of EZH2 and PTEN protein by immunohistochemical SP method. The correlations of EZH2 and PTEN with clinicopathological characters were analyzed by X2 test or Fisher'S exact test; their relationship by Spearman.Results:1. The expression of EZH2 in normal ovarian tissue,benign,border ovarian tumor and ovarian carcinoma Was 5%,33.3%,42.9%,67.3% respectively, and the difference was significant among the four groups(P<0.05).In ovarian carcinoma group, the positive expression of EZH2 in gradeⅠ-Ⅱ(56.7%) Was significantly lower than stageⅢ-Ⅳ(88.9%), the positive expression of EZH2 in no lymph node metastasis group (56.8%) significantly lower than lymph node metastasis (100%), and the differences were both significant (P<0.05). The expression level of EZH2 increased progressively from G1 (42.9%) and G2 (78.3%) to G3 (90.9%), and the difference was significant among the three groups(P<0.05).But no correlation was found between the expression of EZH2 and histological subtype (P>0.05).2.The expression of PTEN in normal ovarian tissue,benign border ovarian tumor and ovarian carcinoma Was 100%,66.7%,42.9%,38.2% respectively, and the difference was significant among the four groups(P<0.05).In ovarian carcinoma group, the positive expression of EZH2 in gradeⅠ-Ⅱ(51.4%) Was significantly lower than stageⅢ-Ⅳ(11.1%), the positive expression of EZH2 in no lymph node metastasis group (51.4%) significantly lower than lymph node metastasis (0%), and the differences were both significant (P<0.05). The expression level of EZH2 increased progressively from G1 (66.7%) and G2 (21.7%) to G3 (18.2%), and the difference was significant among the three groups(P<0.05).But no correlation was found between the expression of EZH2 and histological subtype (P>0.05).3. In ovarian carcinoma group, the expression of EZH2 and PTEN shows the negative correlation. (rs=-0.425, P=0.001).Conclusions:1.The high expression of EZH2 in ovarian cancer has positive correlation with clincal stages,histological grades and lymphaden diversion, indicating that EZH2 protein may be involved in the occurrence of ovarian cancer development, and also indicating that the protein may be associated with invasion and metastasis of ovarian cancer The detection of the protein can be estimated the prognosis of ovarian cancer.2.The low expression of PTEN in ovarian cancer has positive correlation with clincal stages,histological grades and lymphaden diversion,suggesting that the absence of PTEN protein may be associated with ovarian cancer occurrence and development, at the same time indicating that PTEN protein inhibits the invasion and metastasis of ovarian tumor cells。To detect the protein can understand the prognosis of ovarian cancer.3.EZH2 was significantly negative correlation with PTEN, suggesting that the two proteins plays an antagonistic role in the development in ovarian cancer.To detect these molecules simultan-eously, which will be more clinical value for the evaluation of mali-gnant,metastasis and prognosis of ovarian cancer.