Kidney Medicine Abnormal Regulation Of Glucocorticoid-induced Osteoporosis In Rats Adipogenic Differentiation And Lipid Metabolism

Purpose:To explore one molecular mechanism of glucocorticoid induced osteoporosis (GIO) may be the abnormal lipid metabolism and differation; to study the effect of tonifying kidney Chinese herbs on GIO.'Material and method:The GIO model of rats was established by injecting dexamethasone assisted by feeding low-calcium feed. The 143 WISTAR rats were divided randomly into six groups. They are normal group A, model group B, tonifying kidney group C, tonify spleen Chinese herbs group D, Xuefu zuyu group (a medicine used in activating blood to resolve stasis) E and Gushukang group (a medicine used in treating osteoporosis in clinic) F. After nine weeks, we detected the bone density of femur with dual energy X-ray bone density instrument, the expression level of A-FABP mRNA in the femur by real time PCR method and the expression level of A-FABP in the femur by Western blotting method. We also assay the concentration of total cholesterol(TC), triglyceride(TG),high density lipoprotein cholesterol (HDL-C)and low density lipoprotein cholesterol(LDL-C) in serum by automatic biochemistry analyzer.Results:1. Contracted with normal group, the density of femur, the content of TC and HDL-C in model group is markedly reduced(p<0.01), while the expression level of A-FABP and TG in .model group is markedly increased(p<0.01).2. Contracted with model group, the density of femur, the content of HDL-C and TC in tonifying kidney group is markedly increased,while the expression level of A-FABP is markedly reduced(p<0.01).Conclusion:1. By means of injecting dexamethasone, assisted with food control, we have succeeded build up the GIO model of rats.1. One molecular mechanism of GIO may be the abnormal lipid metabolism and differation. 2. By means of tonifying kidney, we find out that a prescription containing Cornu Cervi Pantotrichum, Concha Ostreae and Herba Epimedii Brevicornus can preventing and treating GIO by means of regulating lipid metabolism and differation.