The Effect Of Curcumin On EC-9706 Cells

ObjectivesEsophageal carcinoma(EC) is one of the common malignances in the world. The incidence of EC is high in China, which lines the second in the morbidity of digestive cancer. Researches aiming at treatment of EC are being payed more attention. Therefore, it is very important to search for an effective method to decrease the morbidity and mortality of EC.The mechanism of EC is not completely clear unstill now. The most effective method to treat EC is the surgery at present. Those patients without specific symptoms at early stage had mostly lost the opporunity of surgical therapy, because EC has developed into advanced stage. Therefore, chemotherapy became a key treatment. But chemotherapy is a significant toxicity for patients. The herbal toxicity and adverse reaction of Chinese medicine-curcumin is mild, the price is relatively cheap and it has vast development prospects, which were enrolled in this study. At present, curcumin's anti-tumor mechanism is not very clear. The aim of this study is to investigate that The effect on inhibiting proliferation of EC-9706cells, inducing apoptosis and inhibiting the expressions of BAG-1, HSP70 and HSP27mRNA. In this study, three methods,namely MTT, FCM and RT-PCR, were employed to observe the effect of curcumin on proliferation, apoptosis of EC-9706 cells and expressions of BAG-1, HSP70 and HSP27mRNA, which provide experimental basis for exploring the mechanism of curcumin on EC.Materials and methodsEC-9706 cells were cultured in the environment of 37℃,5%CO2 with routine method. The logarithmically growing EC-9706 cells were used.1 The suppressive effects of curcumin on the proliferation of EC-9706 cells were evaluated in vitro by MTT colrimetric assay2 The apoptical ratio of the EC-9706 cells was evaluated by flow cytometry.3 The expressions of BAG-1, HSP70 and HSP27mRNA were measured by RT-PCR.Results1 EC-9706 cells were divided into three groups:control group, DMSO, and curcumin treatment at the concentration of 50μmol/L,100P<μmol/L,150μmol/L and 200μmol/L for 12h,24h,48h respectively, the growth of cells was significantly inhibited in a time-and dose-dependent fashion. Little influence is found on the growth of cells treated with solvent(P＞0.005).2 The analysis of cellular DNA content by FCM showed that there was a sub-GO/Gl peak in the graph of drug-treated groups. That was a typical apoptotic peak. The alteration occurred in a dose and time dependent manner. After EC-9706 cells were exposed to curcumin(100,150 and 200μmol/L) for 24h, the apoptosis rates were (9.95±1.80)%,(14.58±2.80)%and (40.46±5.87)%respectively, which were significantly higher than control (1.82±0.42)%(P<0.05). The apoptosis rates of 48h were(17.64±1.75)%, (38.22±3.09)%and (48.48±5.56)%respectively, which were significantly higher than control(2.05±0.48)%(P<0.05). 3 Following treating EC-9706 cells with curcumin forl2h,24h and 48h respectively, the expressions of BAG-1, HSP70 and HSP27mRNA were gradually down-regulated with the increasing of exposure time and concentration of it.Conclusions1 Curcumin can significantly inhibit the proliferation of EC-9706 cells in a dose-and time-dependent manner.2 The curcumin can significantly induce the apoptosis of EC-9706 cells in a dose-and time-dependent manner.3 The curcumin can significantly inhibit the expressions of BAG-1, HSP70 and HSP27mRNA, and the expressions of them were gradually down-regulated with the increasing of exposure time and concentration of curcumin.