The Inhibitive Mechanism Of Protocatechuic Acid Against Hepatitis B Virus In Vitro

Protocatechuic acid(PA) has the significantly role of anti-hepatitis B virus. Studies have shown that PA combined lamivudine (3TC) could not only enhance the inhibition of HBV significantly, but also inhibit the HBV X protein entering into the nucleus. But so far, there was no report about molecular mechanism of PA resistance HBV.This paper mainly studied the molecular mechanism of PA resistance HBV in vitro.First of all, after Huh7 human hepatoma cells were transfected with HBV-S1P, HBV-S2P,HBV-CP, HBV-XP, HBV-FP (with two enhancer) promoter-luciferase reporter gene plasmid, and then treated respectively by drugs to analyze the influence of the five HBV gene promoter and the molecular mechanism of PA resistance HBV by drug, Secondly, This experiment had studied the influence of drug to PGL3-NF-kB and PGL3-AP-1 promoter from the angle of signaling pathways and this can help us to explore the correlation between drug and Viral replication and signaling pathways.The results showed that different concentrations of PA has different degrees of inhibition on HBV promoters.PA on the inhibition of FP promoter presented pour u-shaped.When PA is lμg / mL, the inhibition is maximum. And PA inhibited the other four promoters in a dose dependent manner. l0μg/mL of 3TC combined 10μg/mL of PA showed inhibition rate of XP was 59.5%, while the PA was 37.8%.Besides, PA inhibited the AP-1 and NF-kB of pathway promoter also in a dose dependent manner.when the concentration of PA was 10μg/mL,the inhibition rate was biggest, Among them, inhibition rate of AP-1 is 66.4%,and NF-kB is 31.2%.Experimental results indicate that protocatechuic acid on the HBV promoter and promoter signaling pathway inhibition, inhibition of HBV replication is that the drug is one important way for further animal experiments and even provide a theoretical basis for clinical and experimental evidence.