Study On The Correlation Between Immune Function And Chinese Medicine Syndrome Types And Pathological Types In Children With Primary Nephrotic Syndrome

Objectives:1.To study the correlation relationship between T lymphocyte subsets, humoral immune and Chinese Medicine Syndrome Types in children with primary nephrotic syndrome.2.To study the correlation relationship between T lymphocyte subsets, humoral immune and pathologic types in children with primary nephrotic syndrome.3.To study the correlation relationship between T lymphocyte subsets and humoral immune in children with primary nephrotic syndrome.Methods:1.24h proteinuria excretion, plasma cholesterol, plasma albumin, CD3+%, CD4+CD3+%, CD8+CD3+%, CD4+/CD8+, IgG, IgA, IgM, IgE, C3 and C4 are performed in children with primary nephrotic syndrome, whose age are 1 and 18.liver-kidney Yin deficiency syndrome and spleen-kidney Yang deficiency syndrome are selected as the studied subjects, and obtained pathological types by means of kidney biopsy.2.40 patients with primary nephrotic syndrome are divided into liver-kidney Yin deficiency syndrome group (n=19) and spleen-kidney Yang deficiency syndrome group (n=21). The two groups are performed with independent samples group t test based on normal distribution and homogeneity of variance,or performed with two independent samples nonparametric test based on non-normal Distributions or heterogeneity of variance. CD3+%, CD4+CD3+%, CD8+CD3+%, CD4+/CD8+, IgG, IgA, IgM, IgE, C3 and C4 are separately compared.3.35 patients with primary nephrotic syndrome were divided into minimal change disease group (n=18) and mesangial proliferative glomerulonephritis group (n=17), the two goups are performed with independent samples group t test based on normal distribution and homogeneity of variance,or performed with two independent samples nonparametric test based on non-normal Distributions or heterogeneity of variance. CD3+%, CD4+CD3+%, CD8+CD3+%, CD4+/CD8+, IgG, IgA, IgM, IgE, C3 and C4 are separately compared.4.The correlation relationship between CD3+%, CD4+CD3+%, CD8+CD3+%, CD4+/CD8+, IgG,IgA, IgM, IgE, C3 and C4 is evaluated by multiple linear stepwise regressions analysis in children with primary nephrotic syndrome.Results:1.Most of urine protein, plasma albumin and plasma cholesterol in children with primary nephrotic syndrome accord with primary nephrotic syndrome diagnosis criteria.2.Of all the 40 patients in children with primary nephrotic syndrome, liver-kidney Yin deficiency syndrome 19 cases, accounts for 45.0%, spleen-kidney Yang deficiency syndrome 21 cases,accounts for 52.5%. minimal change disease 18 cases, accounts for 45.0%,mesangial proliferative glomerulonephritis 17 cases, accounts for 42.5%, membrane proliferative glomerulonephritis 2 cases, accounts for 5.0%, membranous nephropathy 2 cases,accounts for 5.0%, focal segmental glomerulosclerosis 1 cases, accounts for 2.5%.3.The CD3+% of spleen-kidney Yang deficiency syndrome in children with primary nephrotic syndrome is higher than liver-kidney Yin deficiency syndrome (P<0.05). The CD8+CD3+% of spleen-kidney Yang deficiency syndrome in children with primary nephrotic syndrome is different from liver-kidney Yin deficiency syndrome (P<0.05). Still can't think that CD4+CD3+% and CD4+/CD8+ of liver-kidney Yin deficiency syndrome and spleen-kidney Yang deficiency syndrome in children with primary nephrotic syndrome have the difference.4.Still can't think that IgG, IgA, IgM, IgE, C3 and C4 of liver-kidney Yin deficiency syndrome and spleen-kidney Yang deficiency syndrome in children with primary nephrotic syndrome have the difference.5.Still can't think that CD3+%, CD4+CD3+%, CD8+CD3+% and CD4+/CD8+ of minimal change disease and mesangial proliferative glomerulonephritis in children with primary nephrotic syndrome have the difference.6.Still can't think that IgG, IgA, IgM, IgE, C3 and C4 of minimal change disease and mesangial proliferative glomerulonephritis in children with primary nephrotic syndrome have the difference.7.CD3+% is correlated positively with IgE (r= 0.330).CD4+CD3+% is correlated positively with IgE (r=0.371) and IgM (r=0.482). The correlation relationship between CD4+CD3+% and IgM are stronger than IgE. CD8+CD3+% is correlated positively with IgM (r=0.443). CD4+/CD8+ is correlated positively with IgM (r=0.557)Conclusions:1.The CD3+% of spleen-kidney Yang deficiency syndrome in children with primary nephrotic syndrome is higher than liver-kidney Yin deficiency syndrome. The CD8+CD3+% of spleen-kidney Yang deficiency syndrome in children with primary nephrotic syndrome is different from liver-kidney Yin deficiency syndrome. Still can't think that CD4+CD3+% and CD4+/CD8+ of liver-kidney Yin deficiency syndrome and spleen-kidney Yang deficiency syndrome in children with primary nephrotic syndrome have the difference. 2.Still can't think that CD3+%,CD4+CD3+%,CD8+CD3+%,CD4+/CD8+,IgG, IgA,IgM,IgE,C3 and C4 of minimal change disease and mesangial prolif.erative glomerulonephritis in children with primary nephrotic syndrome have the difference.3.CD3+% is correlated positively with IgE.CD4+CD3+%is correlated positively with IgE and IgM.The orrelation relationship between CD4+CD3+%and IgM is stronger than IgE. CD8+CD3+%and CD4+/CD8+are correlated positively with IgM.