Studies On The Compatibility Of Zhenju Tablets

ZhenJu Tablets(ZJT) compound for the Chinese and Western medicine,from chemical drugs Clonidine(CLO), Hydrochlorothiazide (HYD) and Rutin(RUT) in Chinese medicine, chrysanthemum, pearl powder composition. In this paper,we use the Caco-2 cell model and in vivo intestinal perfusion model to study. The main active ingredient in the prescription of a long intestinal absorption mechanism, in particular the compatibility of Chinese and Western drugs in vivo absorption of drugs.This paper includes three parts of the following contents:1 ZhenJu Tablets main active ingredient of the establishment of DeterminationWere established clonidine, hydrochlorothiazide, rutin and linarin the HPLC determination, and validation of the methodology. Established HPLC methods of CLO and HPLC simultaneous determination of HYD and RUT,and the two methods were methodological validation. The results meet the method requirements, the three components of the cell samples used for determination.2 ZhenJu Tablets main active ingredients of the mechanism of intestinal absorptionCompatibility of Chinese and Western medicine, after oral administration may affect the intestinal absorption process, thus affecting the rate of drug efficacy and extent of play. Intended to Zhenjujiangya the main active ingredients of the film, clonidine, hydrochlorothiazide and rutin absorption rate and absorption capacity index, using in vitro Caco-2 cell culture model of the active ingredient ZJ comprehensive study of absorption mechanisms.Establishment of Caco-2 cell model, and build the model validation by indicators of Morphological examination, measurement of TEER of single-cell layer and so on. Results Caco-2 cell model has good cell morphology and cell integrity is better, can be used for cell uptake and absorption tests.Research ZhenJu Tablets film clonidine, hydrochlorothiazide and rutin and other components of the cellular uptake and transport law, examine P-gp and MRP transporter proteins such as the impact on the absorption process to determine the absorption mechanism. Results CLO and RUT are mainly passive diffusion uptake by Caco-2 cells, there was no efflux function; HYD way of active transport may be cell uptake, but there is the role of efflux protein efflux.3 ZhenJu Tablets major effect of the compatibility of active ingredientsZhenJu Tablets film on the compatibility of different drugs to study the effects of absorption by Caco-2 cell model and in vivo intestinal perfusion model, a single dose of each component separately, combinations between the different components after administration study the absorption process was investigated after application compatibility and the law of absorption, verify ZhenJu Tablets Recipe reasonable. The results of three active ingredients of compound compatibility allows the uptake and transport increased significantly, while the compatibility of each component separately uptake of HYD had no significant effect, CLO can increase the transport capacity HYD; intake of various components were significant for the role of CLO, While no significant increase in transit; and traditional Chinese medicine composition of the uptake and absorption of both RUT have promoted. For perfusion experiments in rat intestinal Caco-2 cells results almost identical results again verify the compatibility of the necessity and rationality ZJT compound.The passage studies on ZJT main active ingredients of the mechanism of intestinal absorption and major effect of the compatibility of active ingredients.The results verified the compatibility of the necessity and rationality ZJT compound.All the dates of the studies make the base of the further research and second study.