| ObjectiveTo investigate whether aerobic exercise could decrease hyperhomocysteinemia, lipid levels and increase SOD activities in ApoE-/-mice by treadmill exercise training. And to provide a reference, which will further develop the prevention and treatment of high homocysteine induced atherosclerosis in an effective way.Method24 Six-week-old female ApoE-/- mice, weighing 16-18g, were randomly divided into three groups: Control group, HHcy group and HHcy+Exercise group. HHcy animal models were fed with a high homocysteine chow (1.8g/L in water). After 1 week of acclimatization, the HHcy+Exercise group was trained in a motorized rodent treadmill for 8 weeks (speed: 15m/min, slope: 0°, 60min/d, 5d/w). Homocysteine levels and lipid levels in plasma were determined enzymatically by auto-biochemistry analysis system. Superoxide dismutase activities were determined by hydroxylamine assay kit.Results1. There were not significant differences in body weight, daily drinking amount, and plasma total cholesterol, LDL-c, HDL-c and triglyceride concentrations between the three groups.2. The level of homocysteine in mice blood in HHcy group were apparently higher than Control group (P=0.001), furthermore, the homocysteine level significantly decreased in HHcy+Exercise group compared with HHcy group (P=0.016).3. Compared with control group, superoxide dismutase activities were lower in HHcy group (P=0.014). However, it was increased in HHcy+Exercise group than HHcy group (P=0.035).ConclusionsAerobic exercise decreased the homocysteine level in plasma and up-regulated the expression of anti-oxidant agent in HHcy ApoE-/- mice, which did not depend on the decrease of cholesterol levels. |
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