Regulation Of IL-6R Antibody On PMMA Bone Cements Mediating Expression Of MMPs And Angiogenic Factors MRNA In Synovial Fibroblasts

Objective: To investigate the expression of MMP-1 and MMP-3, VEGF and PDGF mRNA in synovial fibroblasts regulated by IL-6R antibody mediating on PMMA bone cement.Methods: Synovial tissue obtained from total hip arthroplasty was digestd and subcultured. Inverted microscope was employed to observe the synovial cells and immunocytochemistry (SABC method) staining was used to identified synovial fibroblasts. This experiment was divided into 3 groups according to different cultural conditions: PMMA group: 75 g/ml PMMA bone cements particles; IL-6R antibody group: 10 ng/ml IL-6R antibody+75 g/ml PMMA bone cements particles; control group. Growth inhibition and toxicity of synovial fibroblasts was measured by CCK-8 kit; FQ-PCR was applied to detect the expression of MMP-1, MMP-3, VEGF and PDGF mRNA.Results: The primary synovial cells were forming adherent cells, with the characteristics of short spindle. After 3 consecutive passages, more than 95% of all cells were turning into the fibroblast-like cells. SABC staining results of the fibroblast-like cells showed that anti-CD68 antibody was negative and anti-vimentin antibody was positive, with brown madder stained. CCK-8 test demonstrated that comparing with the PMMA group and control group, absorbance value (read at 450nm) in IL-6R antibody group was significantly lower (P<0.01); while statistical differences of absorbance value (read at 450nm) didn't exist between the control group and PMMA group (P>0.05); Result of FQ-PCR indicated that compared with the PMMA group and control group, expression of MMP-1 and MMP-3, VEGF and PDGF mRNA in IL-6R antibody group was significantly inhibited (P<0.01); and expression of MMP-1 and MMP-3, VEGF and PDGF mRNA in the PMMA group was higher than the control group (P<0.05).Conclusion: IL-6R antibody could significantly inhibit the proliferation and growth of synovial fibroblasts, and moreover, inhibit expression of MMP-1 and MMP-3, VEGF and PDGF mRNA mediated by the PMMA bone cements in synovial fibroblasts, which might provide theoretical basis of molecular biology for the prevention and treatment of aseptic loosening of prosthesis.