Determination Of JYBF Tablet And It's Vivo Analysis

'Jiangya Bifeng'tablet is a new kind of integrated traditional Chinese and Western medicine for the treatment of hypertension. However, up to now, only hydrochlorothiazide was chosen as index component for quality control of JYBF tablet. To date, determination of pargyline in'Jiangya Bifeng'tablet by high performance liquid chromatographic method has been reported, but other methods have not been reported. Determination of active ingredients of'Jiangya Bifeng'tablet and in vivo has not been reported. Studies are as follows:1. Determination of pargyline in'Jiangya Bifeng'tablet by CE-ECLBy optimizing the conditions of CE-ECL, CE-ECL determination of pargyline in 'Jiangya Bifeng'tablet was established based on the optimal sensitization of electrochemiluminescence of ruthenium terpyridine by pargyline. Under the optimimum system, the liner range of pargyline was 0.08-2.0 mg/L, the limit concentration of detection was 1.2μg/L (S/N=3). The RSD of peak height and migration time of 1.2 mg/L pargyline (n=6) was 1.5% and 3.2%, respectively. The study provides a reference for quality control of'Jiangya Bifeng'tablet and other preparations of pargyline.2. Determination of pargyline in rat serum by HPLCBy optimizing the conditions of HPLC, determination of pargyline in rat serum by HPLC as established. As was shown in the result, the concentration of pargyline in rats'serum reached the maximum at 480 min, the parmacokinetics of pargyline showed two-bedroom model and the main parameters were:Ka/h"1=88.097, T1/2α/h=3.343, T1/2β/h=3.663, Cmax/mg L-1=0.4983, AUC(O～t)/mg h L-1=4.523, AUC(0-co)/mg h L-1=5.501. The study provides a reference for parmacokinetics analysis of pargyline.3. Determination of eight active components in'Jiangya Bifeng'tablet by HPLCBy optimizing the conditions of HPLC, determination of eight active components in'Jiangya Bifeng'tablet by HPLC was established. The mobile phase was acetonitrile-0.05 mol/L ammonium dihydrogen phosphate (pH=2.8); the linear gradient elution was:0 min 85%(B),12 min 78%(B),22 min 67%(B),32 min 57% (B); the flow rate was:1.0 ml/min. The study provides a reference for quality control of'Jiangya Bifeng'tablet and other preparations of pargyline.4. Determination of six flavones in rat serum by HPLCBy optimizing the conditions of HPLC, determination of six flavones in rat serum by HPLC was established. As was shown in the result, the C-T profiles of the six flavones in rats'serum showed two-bedroom models after orally administrated 0.5 g 'Jiangya Bifeng'tablet. From the pharmacokinetic parameters, it was seen that the six flavones in rats distribut and eliminate fast, and have broad distributions. The study provides a reference for parmacokinetics analysis of six flavones in'Jiangya Bifeng' tablet.5. Determination of six flavones in rat tissues by HPLCBy optimizing the conditions of HPLC, determination of six flavones in rat tissues by HPLC was established. As was shown in the result, the six flavones were widely distributed in eight tissues after orally administrated 0.5 g'Jiangya Bifeng' tablet. From drug distribution in various tissues, the flavones facilitated diffusion through the gastrointestinal absorption and distribution in the gastrointestinal tract, and in the liver, kidney, heart, lung, spleen, lung, brain and other parts of organization. The study provides a reference for tissue distribution of six flavones in'Jiangya Bifeng' tablet.