Expression Analysis Of Pluripotent Stem Cell-related Genes And Markar Antigens In Different Organizations Dervied From Human And Rhesus Macaque

Recent research showed that some pluripotent stem cells were exited in umbilical cord, umbilical cord blood, amniotic fluid and peripheral blood, but research results are not consistent; Oct-4, Nanog, Sox-2, Rex-1, SSEAs as markers of embryonic stem cells, were often used in evaluation the pluripotency of stem cells. The study on expression analysis of pluripotent stem cell-related genes Oct-4, Nanog, Sox-2 and Rex-1 in human umbilical cord, umbilical cord blood and amniotic fluid by semi-quantitative RT-PCR, and analysis of these genes by homologous; Meanwhile, to test expression and distribution of Oct-4, Nanog, Sox-2, SSEA-1, SSEA-3, SSEA-4 by SP immunohistochemical method, the results were as follows:1> Oct-4, Nanog, Sox-2 and Rex-1 genes expressed stably (positive rate≥80%) in these tissues of umbilical cord, umbilical cord blood and amniotic fluid of human-derived; The expression level of the same gene in above-mentioned tissues is different, no significant difference between the expression level of umbilical cord, Middle and late amniotic fluid (P>0.05), but such is significantly higher than the expression level of umbilical cord blood (P<0.05).2,Oct-4, Nanog and Sox-2 genes expressed stably (positive rate≥80%) in the peripheral blood of different growth stages of physiological state of rhesus macaque; The expression level of the same gene in the peripheral blood of rhesus macaque was negatively correlated with ages, but that had no significant difference (P>0.05).3,Oct-4, Nanog, Sox-2 gene sequences cloned from human umbilical cord, umbilical cord blood, amniotic fluid and rhesus macaque peripheral blood homology comparison of these genes'full-length cDNA sequences from human, rhesus macaque, pig, cow, mouse, brown rat, dog, rabbit and some other species, the results showed that people and rhesus macaque, rabbits, pigs, cattle had a higher homology (75%-95.9%), even with the mice and Rattus norvegicus of the homology was ove 60%; Meanwhile, biological evolution analysis of the genes showed that the genes sequences cloned had closest relationship with the same genes sequence from human and mscaques, the three constituted a closest relationship branch; then with pigs, cattle and dogs make up of a large branch. Secondly, phylogenetic relationships followed by rabbits, rats and mice, etc.4,The positive greater than or equal (++) expression rates of markers including Oct-4, Nanog, Sox-2, SSEA-1, SSEA-3, SSEA-4 is over 75%, and no significant difference for the expression rate between two of the various factors (P>0.05), Oct-4,Nanog and Sox-2 of immunoreactive cells were mainly distributed in the human umbilical cord Wharton's jelly and umbilical vascular around, but, few was nearby endothelial cells.