Changed Levels Of Peripheral Blood EPCs And VEGF, BFGF, ENOS In Rats With Cerebral Ischemic Reperfusion Injury And Their Correlation

ObjectiveTo investigate the Changed levels of peripheral blood endothelial progenitor cells (EPCs) and serum VEGF, bFGF, eNOS in rats with cerebral ischemic reperfusion injury and their correlation. We will obtain some basis for monitoring the degree of the patients with cerebral infarctionMethods50 Sprague-Dawley (SD) rats are randomly divided into the cerebral ischemic reperfusion injury group (15), the normal sham-operation control group (5), the normal group (5), the diabetic cerebral ischemic group (15), the diabetic sham-operation control group (5) and the diabetic group (5) after we selected the healthy SD rats. The diabetic models are induced by streptozotocin (STZ). The cerebral ischemic rats are operated with the thread occlusion method (Longa method). After making the successful model, we took the rats' peripheral blood. The number of peripheral blood EPCs is detected by flow cytometry. The levels of serum VEGF, bFGF, eNOS are detected by enzyme-linked immunosorbent assay (ELISA). First, we adapt the analysis of variance (F test) of single factor to compare the relation of the multiple groups. We adapt the linear correlation to analyse the relation of any of the two variables.ResultsThe number of peripheral blood EPCs and the levels of serum VEGF, bFGF, eNOS were higher in the cerebral ischemic reperfusion injury group (EPCs 5.49±1.80; VEGF 423.15±91.22 pg/ml; bFGF 273.09±36.92 pg/ml; eNOS 3.35±0.68 ng/ml) than the normal group (EPCs 2.42±0.22; VEGF 117.73±15.76 pg/ml; bFGF 85.84±16.91 pg/ml; eNOS 2.01±0.67 ng/ml) and normal sham-operation control group (EPCs 2.51±0.25; VEGF 315.80±44.16 pg/ml; bFGF 126.93±27.35 pg/ml; eNOS 2.30±0.27 ng/ml, the difference was statistically significant (P<0.01) Compared with the normal group, the number of peripheral blood EPCs and the levels of serum VEGF, bFGF, eNOS of the diabetic group (EPCs 1.68±0.11; VEGF 62.61±16.55 pg/ml;bFGF 27.96±9.73 pg/ml; eNOS 0.73±0.07 ng/ml)were decreased, the difference was statistically significant (P<0.05). The number of peripheral blood EPCs was lower in the diabetic cerebral ischemic group (EPCs 0.41±0.23) than the diabetic group, the difference was statistically significant, the difference was statistically significant (P<0.05),but the levels of serum VEGF, bFGF, eNOS in the diabetic cerebral ischemic group (VEGF 110.59±34.26 pg/ml; bFGF 75.85±9.76 pg/ml; eNOS 1.92±0.75 ng/ml) were higher than the diabetic group, the difference was statistically significant (P<0.05). We used the linear correlation to analyse the correlation of the peripheral blood EPCs and the serum VEGF, bFGF, eNOS. The number of peripheral blood EPCs and the levels of serum VEGF have a certain correlation, the difference was statistically significant (P=0.003<0.01); The number of peripheral blood EPCs and the levels of bFGF have a certain correlation, the difference was statistically significant (P=0.001<0.01); But the correlation of the number of peripheral blood EPCs and the levels of serum eNOS have not a statistically significance (P=0.069>0.05).ConclusionAfter cerebral ischemic reperfusion injury of the rats, the number of peripheral blood EPCs and the levels of serum VEGF, bFGF, eNOS would been significantly increased. At the meanwhile, there is a correlation between the number of peripheral blood EPCs and the levels of serum VEGF, bFGF in rats with cerebral ischemic reperfusion injury. Though the correlation of the number of peripheral blood EPCs and the levels of serum eNOS have not a statistically significance, but their tendency of the variation have consistency. So they may affect the pathological and physiological process of the ischemic cerebrovascular disease together through some machanisms, for example, repairing the damaged vascular, protecting the glia and so on. This correlation may has guide meaning for estimating the state of an illness of cerebral infarction, it would provide a new approach to help estimate the degree of stroke and prognosis as well in clinical.