| ObjactiveObservation of Ibutilide, shensongyangxin and combined application of Ibutilide and shensongyangxin to the prevention of rabbit acute atrial fibrillation through Establishing the model of rabbit atrial fibrillation, and exploring the expression ofα1C,β1and HERG and drug intervention.Methods:60 rabbits are Randomly divided into 5 groups:control,stimulated group, Ibutilide group,shensongyangxin group,combined application of Ibutilide and shensongyangxin group,each group has 12 rabbits. Stimulated group:measured with saline(1ml/kg.d).Ibutilide group:measured with Ibutilide(0.1mg/kg.d). Shensongyan-gxin group:given with shensongyangxin(0.2g/kg.d). Combined application of Ibutilide and shensongyangxin group:measured with Ibutilide (0.1mg/kg)and given with shensongyangxin(0.2g/kg). Once a day for one week.Establishing the model of rabbit acute atrial fibrillation:Open thoracic and implant esophagus pacing electrodes into the Left atrium,to stimulate the Left atrium (not including control)。With the same stimulating time(6 hour,whatever success or not) and under the same condition.When operating the surgery,observeing The incidence rate of atrial fibrillation in each group; After operating the surgery, using RT-PCR and Western blot to detect the mRNA and protein expression ofα1C,β1and HERG in the myocardium,and analyze the date.Results:1.The incidence of atrial fibrillation rate in each groups (stimulated,Ibutilide,combined application of Ibutilide and shensongyangxin group) are 75%,41.7%,25%,16.7%.The Ibutilide group,conmbined group is obviously lower than stimulated group (P<0.05), and there is no significant difference between Ibutilide group and combined group (P>0.05). there is no significant difference between Shensongyangxin group and stimulated group (P=0.09). Ibutilide group and Conmbined group is obviously lower than Shensongyangxin group (P<0.05)2.After establishing the model of rabbit arial fibrillation,the The mRNA and protein expression ofα1C and the mRNA expression of (31 in stimulated group, Ibutilide group, Shensongyangxin group and Conmbined group is obviously lower than control (0.68±0.02,0.74±0.02,0.69±0.02,0.73±0.01 vs 0.84±0.01,0.61±0.08,0.69±0.03,0.62±0.02,0.68±0.02 vs 0.79±0.01,0.66±0.01,0.77±0.02,0.67±0.02,0.78±0.02 vs 0.87±0.02) (P<0.01), and there is no significant difference about the mRNA and protein expression of HERG between stimulated group and control and between Shensongyangxin group and control. (0.45±0.02,0.46±0.01,0.46±0.02) (P>0.05), the mRNA and protein expression of HERG in Ibutilide group and Conmbined group is obviously lower than control (0.40±0.01 vs 0.46±0.02,0.41±0.01 vs0.46±0.02) (P<0.01)3. The mRNA and protein expression ofα1C and the mRNA expression ofβ1 in Ibutilide group is higher than stimulated group (0.74±0.02 vs 0.68±0.02,0.69±0.03 vs 0.61±0.08,0.77±0.02 vs 0.66±0.01) (P<0.01), the mRNA and protein expression of HERG in Ibutilide group is lower than stimulated group (0.40±0.01 vs 0.45±0.02,0.49±0.01 vs 0.61±0.02) (P<0.01)4. There is no significant difference about the mRNA and protein expression ofα1C and the mRNA expression of (31 between shensongyangxin group and stimulated group (0.69±0.02 vs 0.68±0.01,0.62±0.02 vs 0.61±0.08,0.67±0.02 vs 0.66±0.01) (P>0.05),and in this two group, the mRNA and protein expression of HERG has no change (0.46±0.01 vs 0.4±0.02,0.60±0.02 vs 0.61±0.02) (P>0.05)5. The mRNA and protein expression ofα1C and the mRNA expression ofβ1 in Ibutilide group in higher than shensongyangxin group (0.74±0.02 vs 0.69±0.02,0.69±0.03 vs 0.62±0.02,0.77±0.02 vs 0.67±0.02) (P<0.01), the mRNA and protein expression of HERG in Ibutilide group in lower than shensongyangxin group (0.41±0.01 vs 0.46±0.01,0.49±0.01 vs 0.60±0.02,) (P<0.01)6. The mRNA and protein expression ofα1lC and the mRNA expression ofβ1 in Combined group is higer than stimulated group(0.73±0.01 vs 0.68±0.02,0.68±0.02 vs 0.61±0.08,0.78±0.02 vs 0.66±0.01) (P<0.01), the mRNA and protein expression of HERG in Combined group is lower than stimulated group(0.41±0.01 vs 0.45±0.02,0.50±0.01 vs0.61±0.02) (P<0.01);There is no significant difference about the mRNA and protein expression ofα1C and the mRNA expression ofβ1 between Combined group and Ibutilide group ((0.73±0.01 vs 0.74±0.02,,0.68±0.02 vs 0.69±0.03,0.78±0.02 vs 0.77±0.02) (P>0.05),and the same to the the mRNA and protein expression of HERG (0.41±0.01 vs0.40±0.01,0.50±0.01 vs 0.49±0.01) (P >0.05); The mRNA and protein expression ofα1C and the mRNA expression ofβ1 in Combined group is higer than Shensongyangxin group (0.73±0.01 vs 0.69±0.02,0.68±0.02 vs 0.62±0.02,0.78±0.02 vs 0.67±0.02) (P<0.01), the mRNA and protein expression of HERG in Combined group is lower than Shensongyangxin group (0.41±0.01 vs 0.46±0.01,0.50±0.01 vs0.60±0.02) (P<0.01)Conclusion:1.Ibutilide and combined application of Ibutilide and shensongyangxin could prevent rabbit acute atrial fibrillation, but there is no significant difference between Ibutilide and Combined group in preventing acute rabbit atrial fibrillation.2. shensongyangxin has no obvious effect of preventing rabbit acute atrial fibrillation,but combined application with ibutilide,it's opposite.3.After atrial rapid stimulating,the expression ofα1C andβ1 in myocardium in stimulated group is obviously lower than the control, and the expression of HERG have no change. 4.1butilide could increase the expression ofα1C andβ1,and reduce the expression of HERG.5. shensongyangxin cannot increase the expression ofα1C andβ1,and has no effect on the expression of HERG.6. The expression ofα1Cβ1 and HERG in myocardium have no obviously change between Ibutilide and Combined group. |
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