The Experimental Studies Of Protection Role Of Recombinant Human Erythropoietin On Brain In The Septic Shock Rats

ObjectiveTo study the protection role of recombinant human erythropoietin (rhEPO) on brain in the septic shock rats.Method30 rats were randomly divided into three groups:control group(n=6), septic shock group(n=12) and rhEPO group(n=12). The rats of septic shock group and rhEPO group were randomly divided into two time points:6h and 12h, and they were established the models of tail intravenous endotoxin in 5mg/kg doses, in the rhEPO group when the mice appeared shock signs intraperitoneal injection of rhEPO in 5000U/kg doses immediately. And the mice in the control group were only injected amount saline. After surgery all the rats were partial anesthesiaed and eye removal to take the blood to detect the concentration of NSE, then breakaged the mice to get the brain to detect the concentration of NO.6 mice of each group were executed to extract the sample for the pathological section at the different time points, and the sections were stained with Hematoxylin and Eosin and TdT-mediated dUTP nick end labeling.The number of each slice about TUNEL were counted by two independent observer with a average number in 5 random high power fields.Result1,Hematoxylin and Eosin stainHistopathological examination showed:the neurons cells of control group were normal,3-4 layer vertebral somatic closely aligned in hippocampal region, the nuclear of neurons is big round and transparent, having 1-2 prominent nucleoli in high power fields. The vertebral somatic of septic shock were degeneration, nuclear become pyknotic, hyperchromatic and intercellular increase. The survival vertebral somatic in hippocampal region of rhEPO group increasing, damage degree were significantly reduce. 2,TdT-mediated dUTP nick end labeling stainTUNEL-positive cells were not seen in the control group. Many TUNEL-positive cells were seen in the septic shock group with 12h more serious. The TUNEL-positive cells of rhEPO were significantly reduced.3,Brain homogenate NO determinationThe level of NO in brain tissue of the control group was (2.72±0.24)μmol/g.prot, compared with that in the control group, the level of NO in brain tissue of the septic shock group increased significantly with 12h more serious, to (6.35±0.41)μmol/g.prot. The level of NO in brain tissue of the rhEPO group was greatly decreased compared with the septic shock group.4,Serum NSE determinationThe level of NSE of the control group was (26.26±2.63)μg/ml, the level of NSE of the septic shock group increased significantly with 12h more serious, to (52.42±3.65)μg/ml. The level of NSE of the rhEPO group was greatly decreased compared with the septic shock group.Conclusion1,rhEPO can improve shock signs of the model of rats.2,rhEPO can reduce the concentration of NO in brain tissue and NSE of serum.3,rhEPO can reduce apoptosis in neurons of hippocampal region.4,rhEPO have a neuroprotective effect on brain in the septic shock rats.