Study On The Formulations Of Solid Lipid Nanoparticles For Calciporitol

Solid lipid nanoparticles is a promising drug delivery system with solid biodegradable lipid as drug carriers, diameters ranging between 50 and 1000 nanometers, which has the advantages of high physical stability, slow speed of drug leakage and low toxicity etc. the object of this thesis was to study the preparation, characterization, in vitro release and in vivo behavior of CPT-SLN.The thesis was to study of SLN with calcipotriol as a model drug. A new method named flocculation and filtration was set up to separate free calcipotriol from that incorporated in the SLN to determine the entrapment efficiency of calcipotriol in the SLN. The method could be easily operated with accurate results. A series of CPT-SLN were prepared by a nanoemulsion technique employing high-speed homogenizer and ultrasonic probe. Many physico-chemical characteristics of CPT-SLN were investigated. The appearance of the CPT-SLN was examined by transmission electron microscopy; particle size and Ploydispersity index (PDI) were evaluated by Zetasizer; X-ray, DSC and FT-IR study showed that the calcipotriol encapsulated in SLN was in a new form; the long-term stability was also evaluated.The results showed that the appearance of CPT-SLN was spherical with mean particle size below 200nm, PDI between 0.1 and 0.5, zeta potential-25mv--17mv. The entrapment efficiency was above 90%. The stability experiments indicated that the CPT-SLN could be kept stable at 4℃, away from light for 3months. Compared to the DOVOBET ointment, the CPT-SLN gel had fewer transdermal penetration and more accumulation of skin. It indicated that SLN can raise the accumulation of skin remarkably and reduce the transdermal penetration to blood markedly.The results of preliminary pharmacodynamics showed that the CPT-SLN gel had more effective anti-psoriasis than DOVOBET ointment.