Correlation Between Expression Of MMP-2,TIMP-3 And CD44v6 In Gastric And Its Clinical Significace

Objective:Gastric cancer is the most common malignant tumor of digestive tract in our country, and the annual number of death is highest than all the other carcinomas. Invasion and metastasis are the most important biological behavior of carcinoma, which are also the chief reasons of recurrence and death. Thus, investigating the factors which can influence the invasion and metastasis of gastric cancer and persuing the biological markers for determining the malignant behavior of gastric cancer with important implications.Lots of studies manifest that the imblance of MMP-2 and TIMP-3 expression and the overexpression of CD44v6 in gastric cancer are eminent correlative with invasion and metastasis. Expression of MMP-2, TIMP-3 and CD44V6 were detected by immunohistochemical method in normal gastric mucosa, dysplastic gastric mucosa and gastric cancer tissues, to investigate their correlativity and the effect to the biological behavior of gastric cancer.Methods:30 samples of gastric cancer that were amputated in Zhangshan hospital Affiliated to Dalian University from 1,2008 to 12,2009 were studied, including 21 males and 9 females.The median age is 59.31. All cases of gastric cancer did not accepted the radiotherapy and other pharmacotherapy, and 15 samples of dysplastic gastric mucosa and 15 samples of normal gastric mucosa were screened randomly in control group. Expression of MMP-2, TIMP-3 and CD44v6 were detected by immunohistochemical method in 60 samples of gastric tissues.Results:In the development of normal gastric mucosa→dysplastic gastric mucosa→gastric cancer tissues, the positive overexpression rates of MMP-2, TIMP-3 and CD44V6 proteins all showd rising trend. The difference of MMP-2 and CD44V6 expression in gastric cancer tissues versus normal gastric tissues, and gastric cancer tissues versus dysplastic gastric tissues was statistically significant, while there was no evident difference of MMP-2 and CD44v6 expression between dysplastic gastric tissues and normal gastric tissues. The difference of TIMP-3 expression in gastric cancer tissues versus normal gastric tissues was statistically significant, while there was no evident difference of TIMP-3 expression between gastric cancer tissues and dysplastic gastric tissues, and also between dysplastic gastric tissues and normal gastric tissues.The expression of MMP-2, TIMP-3 and CD44V6 protein in gastric cancer tissues was not related with sex and age. The expression of MMP-2 and CD44V6 protein was positive related with the differentiation, depth of invasion, lymph node metastasis, distant metastasis, and TNM stage. There was a positive correlation between the MMP-2 expression and the CD44V6 expression. The expression of TIMP-3 was negative related with depth of invasion, lymph node metastasis, and TNM stage, while there was no correlativity with the differentiation and distant metastasis. There was inverse correlation between the MMP-2 expression and the TIMP-3 expression in gastric cancer tissues.Conclusion:Detecting MMP-2, TIMP-3 and CD44V6 the protein expression in Gastric Cancer may be helpful to judge the degree of malignancy and potential lymph node metastasis and evaluate the prognosis.