The Effect Of The Inhibitor Of Cyclooxygenase-2 Combined With Radiotherapy On Proliferation Of Cervical Cancer Hela Cells

Objective: To observe the effect of selective cyclooxygenase-2 inhibitor celecoxib on growth in cervical cancer Hela cells. To study the radiosensitivity of celecoxib on human cervical cancer Hela cell line and the influencing factor schedule. The possible mechanisms of radiosensitization were investigated in this study.Methods:The inhibitory effect of selective COX-2 inhibitor on the growth of human cervical cancer Hela cell line was observed in vitro by MTT method.The cytoto-xicity of celecoxib on Hela cells was measured by MTT assay.Measured by colony formation assay cells radiosensitivity parameters: the average lethal dose (Do), Quasi-field dose (Dq),2Gy irradiation of the surviving fraction (SF2) and radiosensitization ratio (SER);Hela cell was treated in vitro with different doses of radiation alone or radiation with drug by various schedules,Colony forming assaying and single-hit muhitarget model were used to perform survival fraction analysis and plotting survival curve and sensitive enhancement ratio(SER).The effects of celecoxib on the expression of cyclooxygenase-2,VEGF,VEGF-C,antigen were analyzed by immunohistochemistry.Results 1.MTT study showed that selective COX-2 inhibitor produced a dose-dependent and a time-dependent inhibition of Hela proliferation: Compared with the control group, the difference was statistically significant (P<0.05). The IC50 was 44μmol/L.2. Logarithmic phase Hela cells were divided into 3 groups, the negative control group were culture medium, radiotherapy alone group was 6 MVX ray radiation therapy, radiation plus drug group first with 20 and 40μmol/L of celecoxib, 72 h, re-irradiation. Radiation plus drug group Do,Dq and SF2 were less than the control group radiation alone (P all <0.05), its SER increased from 2.01 to 2.41.3. The IC50 of celecoxib was 44μmol/L.The SER of 2.41, 1.89 and 1.44 was observed when radiation was given prior to, concurrently with or after treatment with 40μmol/L celecoxib.4. Immunohistochemistry revealed that celecoxib down-regulated the expression of COX-2,VEGF,VEGF-C on Hela cells. The expression of COX-2,VEGF,VEGF-C was significantly decreased in radiotherapy plus drug group(Celecoxib, 40μmol/L)Hela cells (P all <0.05), compared with the negative control group and radiotherapy alone.Conclusion: Celecoxib can increase the radiosensitivity of cervical cancer Hela cells, which might be related to the influence of celecoxib down-regulated the COX-2,VEGF-C expression of Hela cell.This may be provide a new clinical method of cervical cancer treatment.