Protective Effects Of Hyperoxygenated Solution By Intravenous Injection On Multiple Organ Damage Of Rats With Acute Carbon Monoxide Poisoning

Pure CO (carbon monoxide, CO) belongs to the organics intermediates in burning and oxidation. It is colorless, odorless, non-irritating poisonous gas, its molecular weight is 28, its density is 0.967g / L and its solubility in water is low. When CO is breathed into body, it will immediately and tightly reversible bind to hemoglobin. Hb subsequently loss the normal function of oxygen carrying, causing the tissues and organs appear hypoxia. Without timely discovery and effective treatment, it will eventually lead to suffocation. Acute CO poisoning caused the most fatal accidents at home and works in the world. Therefore, it has very important social meaning to explore the prevention method of CO poisoning with higher clinical value.ObjectiveBuilt CO poisoning rat model by intraperitoneal injection (ip), investigate the therapic effects of hyperoxygen solution (HOS) in different doses on the model, verify its protective effects on important organ by pathology and biochemical parameters and provide a reference for clinical application. M ethods30 SD rats were randomly divided into 5 groups, 6 rats in each group. Normal group (N group), carbon monoxide poisoning group (C group) and different dose of HOS treatment groups at 1h after CO poisoning, 10ml/kg (H10 group), 15ml / kg (H15 group) and 20ml/kg (H20 group). CO poisoning model were established by intraperitoneal injection CO with 120ml/kg. C group and the treatment group used such model rats, while N group were given the same dose of air. All the rats were collected 0.5ml of arterial blood for blood gas measurement and 3 ml of venous blood for the determination of sensitive biochemical indexes in brain, heart, liver, and kidney. At the end of treatments, all animals were infused 500ml normal saline and 4% paraformaldehyde from the left ventricle in narcosis, and then taken the same biopsy site of heart, brain, liver, lung and kidney to make pathological section and observe its results under light microscope.R esultsCO (120ml/kg in ip 1.5h later) cause severe hypoxemia. PaO2 decreased from the 96.62±2.4 mmHg to 43.04±4.13mmHg. After CO poisoning for 1d, C group had significantly increase in Neuron-specific enolase (NSE), troponin (cTnT), creatine kinase (CK), creatine kinase MB (CK-MB), lactate dehydrogenase (LDH ),α-hydroxybutyrate dehydrogenase (α-HBDH), total bilirubin (TBIL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamyl transferase (GGT), blood urea nitrogen (BUN ) and creatinine (Cr) (P <0.01). After CO poisoning for 24h, brain cells showed edema, interstitial widening; myocardial cells showed continuity incomplete, ill-defined and vacuolar degeneration; liver cells were arranged in disorder and vacuolar degeneration; renal interstitial showed edema and cell boundary is unclear and a large number of red blood cells and inflammatory cells had emerged; alveolar structural disorder, edema, a large number of red blood cells and inflammatory cells had emerged in interstitial.When intravenous infused with different doses of HOS, Dissolved oxygen in plasma was significantly increased, PaO₂ increased from the 43.04±4.13 mmHg to 81.65±3.85mmHg.all the enzymes were significantly lower than these in C group, although still were significantly higher than these in N group(P<0.05 or P<0.01). Moreover, all the enzymes were significantly lowest in group H20 with a significantly dose dependent relationship.Conclusionâ‘ Built a CO poisoning rat model by 120ml/kg in ip, which is Simple, reliable, reproducible.â‘¡compared with CO poisoning group, hyperoxygenated solution (HOS) by intravenous significantly increased the PaO₂ and SaO₂, significantly decreased the to the biochemical indexes and significantly alleviated the damage of important organs. In short, hyperoxygen solution (HOS) has significantly prevented the hypoxia damages to importance organs from CO poisoning in a dose-dependent manner.