The Study On IGF-1 Promoter Region CA Repeat Allele Polymorpism And The Risk Of Retinopathy Of Prematurity

Objective By study the relation between insuline-like growth factor-1(IGF-1)(CA) repeat allele and retinopathy of prematurity, to discuss the effection of IGF-1 (CA) repeat allele on retinopathy of prematurity. Material and Methods Collected the clinical date and blood sample of the patients who had retinopathy of prematurity in Guangdong Women and Children Hospital and Guangzhou Women and Children Therapy Center from 2010.1 to 2011.1. Include 36 infants are ROP group. Design control group in Guangdong Women and Children hospital, according the number(49 infants). Venous blood samples (1 ml) were taken, extract DNA sample, by sequencing, account CA repeat allele number, homozygotes and heterozygotes. By compare the CA repeat allele polymorphism and homozygotes or heterozygotes state difference between control group and ROP group, to discuss the effect of IGF-1 on retinopathy of prematurity.Result1.IGF-1(CA) 17, 18, 19, 20, 21, 22, 23 repeat allele were found in the research group, the most one is (CA) 19 repeat allele, (CA) 21 repeat allele is the second.2. In the risk analyse between IGF-1 genotype and ROP, who carry IGF-1(CA) 17, 19, 21 repeat allele ,the OR(95%CI) is 1.92(0.40-9.15), 1.32 (0.53-3.30)and 1.33(0.56-3.16) respectively, P >0.05.3. In the correlation analyse, no statistically significant associations between the IGF-1 genotype and ROP, P >0.05.ConclusionThe effection of IGF-1 (CA) repeat allele on retinopathy of prematurity has no found.