The Study Of CCK-8S On Behavior And Its Mechanisms Included By Glutamat Meutrasmtw System In Acholoic Rats

Ⅰ. The effects of CCK-8S on memory and voluntary behavior of acute ethanol induced rats.Objective: To establish ethanol induced rat model, we observed the effects of ethanol on memory and voluntary behavior of rats, the changes of memory and voluntary behavior after CCK-8S used in order to discuss the possible mechanism of the effects of CCK-8S on memory and voluntary behavior of acute ethanol induced rats.Methods: the rats were randomly divided into 11 groups (n=8):group A: 10 ml/kg 0.9% normal saline intragastric administration group; group B:10 ml/kg 0.9% normal saline intragastric administration+10 ml/kg 0.9% normal saline abdominal injection group; group C: 10 ml/kg 50% ethanol intragastric administration; group D:low-dose 4μg/kg CCK-8S abdominal injection: group E: low-dose 4μg/kg CCK-8S abdominal injection group+ethanol intragastric administration; group F: high-dose 10μg/kg CCK-8S abdominal injection; group G:high-dose 10μg/kg CCK-8S abdominal injection group+ethanol intragastric administration; group H: CCK-8S inhibitor and low-dose CCK-8S abdominal injection and ethanol intragastric administration group; group I: CCK-8S inhibitor and high-dose CCK-8S abdominal injection and ethanol intragastric administration group; group J:NMD A receptor inhibitor abdominal injection group; group K: NMDA receptor inhibitor abdominal injection and ethanol intragastric administration group; we observed the voluntary changes of rats after ethanol intake and tested the memory before and after ethanol dependence using water gaze method.Results: 1. the rats voluntary activities were increased after great amount of ethanol in short-term period with 200s; 2. the time of clamping of the rats were prolonged in ethanol induced rats; 3. the frequency of voluntary activities of acute ethanol induced rats were decreased and the time of clamping was shortened after using CCK-8S(P<0.05).Conclusions: CCK-8S can decrease the frequency of voluntary activities of acute ethanol rats and improve the memory.Ⅱ. The effects of CCK-8S on expression of NMDA receptor subunits in hippocampus of acute ethanol induced rats.Objective: To establish ethanol induced rat model, we observed the expression of NMDA receptor subunits in hippocampus, the changes of memory and voluntary behavior after CCK-8S used in order to discuss the possible mechanism of the expression of NMDA receptor subunits in hippocampus of acute ethanol induced rats.Methods: the rats were randomly divided into 11 groups (n=8):group A:10 ml/kg 0.9% normal saline intragastric administration group; group B:10 ml/kg 0.9% normal saline intragastric administration+10 ml/kg 0.9% normal saline abdominal injection group; group C:10 ml/kg 50% ethanol intragastric administration; group D:low-dose 4μg/kg CCK-8S abdominal injection: group E:low-dose 4μg/kg CCK-8S abdominal injection group+ethanol intragastric administration; group F: high-dose 10μg/kg CCK-8S abdominal injection; group G: high-dose 10μg/kg CCK-8S abdominal injection group+ethanol intragastric administration; group H:CCK-8S inhibitor and low-dose CCK-8S abdominal injection and ethanol intragastric administration group; groupⅠ: CCK-8S inhibitor and high-dose CCK-8S abdominal injection and ethanol intragastric administration group; group J: NMDA receptor inhibitor abdominal injection group; group K: NMDA receptor inhibitor abdominal injection and ethanol intragastric administration group; we tested the memory before and after ethanol dependence using water gaze method and then obtained the hippocampus tissue to detect the expression changes of mRNA of NMDA receptors by using RT-PCR technique.Results: 1. the expression of mRNA of NMDA receptors in hippocampus of ethanol induced rats was increased; 2. the expression of mRNA of NMDA receptors in hippocampus of ethanol induced rats was increased after using CCK-8S; 3. the expression of mRNA of NMDA receptors in hippocampus of ethanol induced rats was increased after NMDA receptor inhibitor administration.Conclusions: CCK-8S can increased the expression of mRNA of NMDA receptors in hippocampus of ethanol induced rats.