The Expression And Significance Of Tim-3-Gal-9 Pathway In Murine Rsa Models

【Objective】The expression of Tim-3 and Gal-9 in the trophoblast cells of murine RSA and normal pregnant models was detected by immunocytochemical assay, for the sake of exploring the relation between Tim-3-Gal-9 pathway and recurrent spontaneous abortion.【Methods】1. Female CBA/J mice were mated with male Balb/c and DBA/2 mice respectively to construct normal pregnant and spontaneous abortion models.2. The immunocytochemical assay was used to measure the expression of Tim-3 and Gal-9 in the trophoblast cells of murine RSA and normal pregnant models, and then a highly precise color-image measure system was applied to a statistical analysis. 10 visual fields of the high power lens were chosen in every case, and then the mean optic densities were calculated where Gal-9 was positive. 【Results】1. The normal pregnant and spontaneous abortion models were successfully constructed.2. Cytoplasm of trophoblast cells both in normal pregnant and RSA models turned into brown, which suggested Tim-3 was positive in the trophoblast cells. The level of Tim-3 in RSA models was significantly lower than that in normal pregnant models(P <0.05).3. Gal-9 was positive in the trophoblast cells both of normal pregnant and RSA models. Compared to normal pregnant models, the level of Gal-9 in RSA models was significantly lower than that in normal pregnant models(P <0.05).【Conclusion】Tim-3 and Gal-9 were expressed in the trophoblast cells of normal pregnant and RSA models. The abnormal expression of Tim-3 and Gal-9 may result in RSA. The expression of Tim-3-Gal-9 pathway in placental tissues【Objective】The expression of Tim-3 was detected by immunohistochemical assay in the placental tissues of normal pregnant and RSA models, and Gal-9 was detected by immunohistochemical assay and western blot in order to explore the relation between Tim-3-Gal-9 pathway and recurrent spontaneous abortion.【Methods】1. Immunohistochemical assay was used to measure the expression of Tim-3 and Gal-9 in the placental tissues of normal pregnant and RSA models, and then a highly precise color-image measure system was applied to a statistical analysis. 10 visual fields of the high power lens were chosen in every case, then the mean optic densities were calculated where Tim-3 and Gal-9 was positive .2. Western blot was applied to the detection of Gal-9 in placental tissues of normal pregnant and RSA models, and then a statistical analysis was needed.【Results】1. Tim-3 was positive in the placental tissues both of normal pregnant and RSA models. Compared to normal pregnant models, the level of Tim-3 in RSA models was significantly higher(P <0.05).2. Gal-9 was expressed in the placental tissues both of normal pregnant and RSA models. The level of Gal-9 in the placental tissues of normal pregnant and RSA models was significantly lower than that in normal pregnant models.【Conclusion】Tim-3 and Gal-9 were expressed in the placental tissues of normal pregnant mice, but the dysfunction of Tim-3 and Gal-9 pathway may be related to RSA.