The Relationship Between CYP1A1,GSTT1 And GSTM1 Genetic Polymorphisms And Susceptibility Of Ningxia Hui People Esophageal Carcinoma

Objective Esophageal cancer (Esophageal Cancer, EC) is one of the most common gastrointestinal tumors in China, the mortality rate ranked fourth in malignant tumors. Ningxia is one of the areas of high incidence of esophageal cancer, the high incidence of esophageal cancer in line with the typical multi-gene interaction between genetic susceptibility and environmental disease model. The topics chosen for the Msp1 site of CYP1A1, GSTT1, GSTM1 gene research, aimed at discovering CYP1A1, GSTT1, GSTM1 gene polymorphism and esophageal cancer susceptibility in Ningxia Hui, Hui population in Ningxia region to understand the genetic mechanism of esophageal cancer provide some theoretical basis for the realization of susceptible population in Ningxia Hui early esophageal cancer prevention, early diagnosis and early treatment provide a reference.Method 1. Experimental Methods: 40 cases of Ningxia Medical University Hospital diagnosed as esophageal cancer by surgery and pathology Hui people paraffin tissue sections inpatients and 80 healthy Hui people blood samples of blood donors , resin-based DNA extraction kit extracted DNA, PCR amplification of specific fragments, restriction enzyme digestion, electrophoresis. 2. Statistical Methods: 1. Application of t test analysis of the case group and control group differences in age, whether the balance.χ2 test analysis between cases and controls the frequency of genotype, sex, smoking and alcohol consumption is statistically significant and CYP1A1, GSTM1, GSTTl gene polymorphism and esophageal cancer susceptibility and calculate the odds ratio (oddsratio, OR) and 95% confidence interval (95% Confideneeinterval, 95% CI). Result 1. Esophageal cancer group and control group for age, sex, smoking, alcohol consumption was no significant difference (P> 0.05); 2.CYP1A1 MSP1 locus genotypes of the three genotype TT (P = 0.78> 0.05 ), TC (P = 0.37> 0.05), CC (P = 0.16> 0.05) in the esophageal cancer group and control group, no significant difference in the distribution. But the CC genotype (OR = 2.032,95% CI = 0.751-5.496) showed higher tendencies. ; 3. GSTT1 (-) genotype (P = 0.012 <0.05, OR = 0.372,95% CI = 0.170-0.813) that GSTT1 deletion in the esophageal cancer group and control group were significantly different. 4. GSTM1 (-) genotype (P = 0.897> 0.05), that lack of GSTM1 genotype in esophageal cancer group and control group, no significant differences in the distribution. 5. T1 (-) M1 (+) (P = 0.007 <0.05, OR = 3.378,95% CI = 1.360-8.386) and T1 (+) M1 (+) (P = 0.047 <0.05, OR = 0.354,95 % CI = 0.123-1.017) in the esophageal cancer group and control group differences in the distribution. 6.CYP1A1, GSTT1 and GSTM1 combined genotypes in esophageal cancer group and control group no significant difference in the distribution. However, T1 (-) M1 (-) tc / cc combined gene in esophageal cancer group showed higher tendency (OR = 2.212,95% CI = 0.718-6.817).Conclusion 1.CYP1A1 the Msp1 polymorphism alone can not lead to the occurrence of esophageal cancer. 2.GSTT1 (-) genotype is the Ningxia Hui risk factors for esophageal cancer. 3.GSTT1 (+) GSTM1 (+) in the esophageal cancer group and Hui Hui in the distribution of the normal control group, significant differences in the incidence of esophageal cancer with GSTT1 and GSTM1 genotypes may be missing relevant.