The Preliminary Study Of PK2 Expression In Human Testis And Its Potential Funtion In Spermiogenesis

Objective: To investigate the distribution of PK2/BV8 protein in human testicular tissue.Method: Testicular tissue samples were divided into normal group and the non-obstructive azoospermia group. The normal control group was 4 cases normal testicular tissue of autopsy. The non-obstructive azoospermic group testicular tissues were obtained from 6 patients who had undergone testicular biopsy in our hospital due to infertility during 2008-2009. Double-labeled immunofluorescence was used for the study of PK2/BV8 protein distribution in human testis.Result: PK2/BV8 protein is mainly expressed in Leydig cell.Conclusion: The expression of PK2/BV8 protein in human Leydig cells provided evidence for further study of the roles of PK2/BV8 in Leydig cells.Objective :To examine the differences of PK2/BV8 protein and its mRNA expression level between the normal testis and non-obstructive azoospermic testis, and to provide experimental basis for further research of the role the PK2/BV8 playing in spermatogenesis.Method: According to part I, testicular tissue samples were divided into normal group and the non-obstructive azoospermia group. The control group was normal testicular tissue of autopsy for 4 cases. The non-obstructive azoospermic group testicular tissues were obtained from 6 patients who had undergone testicular biopsy in our hospital due to infertility during 2008-2009. The mRNA and protein expression of PK2/BV8 in human testis (normal and non-obstructive azoospermic testicular tissues) was investigated by real time RT-PCR and Western blot,β-Actin was used as internal control.Result: Relative low level of PK2/BV8 mRNA and protein were detected in testis with non-obstructive azoospermia. The mRNA and protein expression of PK2/BV8 had similar tendency in human testis (normal and non-obstructive azoospermic testicular tissues).Conclusion: The expression level of PK2/BV8 decreased in non-obstructive azoospermia testicular tissue suggested that PK2/BV8 may be involved in spermatogen.