The Correlative Analysis Between Auto-antibodies Against AT1-Receptor,Autoantibodies Aganinst Aplhal-adrenergic Recetpor And Endothlial Micropartles In Patients With Gradc Three Hypertension

Objective To investigate the effects of extreme blood pressure on circulatingendothelial microparticles in patients with grade three hypertension.Methods Twenty-six normotensives and twenty-three patients with grade threehypertension were recruited for participation in this study.The circulating endothelialmicroparticles(EMP) were detected with flow cytometry. Blood urea nitrogen (BUN),creatinine(Cr), urine protein, blood cholesterol(TC), low-density lipoprotein (LDL-C)were examined in each group. Body mass index (BMI) was calculated. Factors thatmay influence the EMP levels were analyzed.Results (1)The circulating endothelial microparticles level was higher in grade threehypertension than in control(506.91±322.47 vs 285.81±208.32 , P<0.05);(2)UsingEMP as the dependent variable seperately, multiple linear regression analysis showedthat EMP had a linear relation with SBP, DBP and smoking(R2=0.413, P＜0.05).Conclusion The findings showed that the circulating endothelial microparticles levelwas higher in patients with grade three hypertension than in normotensives. Extremeblood pressure might play a role in the produce of EMP and EMP may be a marker ofthe effects of blood pressure on the endothelium. Objective To investigate the effects of autoantibodies against AT1-receptor,autoantibodies against alpha1-adrenergic receptor on circulating endothelialmicroparticles in patients with grade three hypertension.Methods Totally sixty-seven patients with grade three hypertension were enrolled inthis study(not exclude subjects with Type 2 Diabetes Mellitus, dyslipidemia, et).Based on the existence or non-existence of the autoantibodies, the subjects weredivided into two further groups in two ways: autoantibodies against AT1-receptorpositive group(n=23) and autoantibodies against AT1-receptor negative group (n=44);Or autoantibodies against alpha1-adrenergic receptor positive group(n=18) andautoantibodies against alpha1-adrenergic receptor negative group(n=49). The epitopeof the 2nd extracellular loop of type 1 angiotensin (AT1) receptor andalpha1-adrenergic receptor were synthesized and used as antigens to screen theautoantibodies against AT1-receptor and alpha1-adrenergic receptor by ELISA. Thecirculating endothelial microparticles(EMP) were detected by flow cytometry.Detect the other biochemical markers using the same method as above.Results (1)The circulating endothelial microparticles level was higher inautoantibodies against AT1-receptor positive group than in autoantibodies againstAT1-receptor negative group(575.70±278.47 vs 433.65±194.12, P<0.05); (2)EMP didnot differ between autoantibodies against alpha1-adrenergic receptor positive groupand autoantibodies against alpha1-adrenergic receptor negative group(518.29±358.21vs 526.06±265.58, P>0.05).(3)Multiple regression analysis was applied to evaluateclinical and biochemical variables age, gender, BMI, smoking, diabete, BUN, Cr, urine protein, TC, LDL-C, autoantibodies against AT1-receptor and autoantibodiesagainst alpha1-adrenergic receptor as independent variables in regression models ofthe dependent variables EMP. Multiple linear regression analysis showed that EMPhad a linear relation with SBP, DBP and autoantibodies againstAT1-receptor(R2=0.413, P＜0.05).ConclusiConclusion The findings showed that patients with the activity of autoantibodiesagainst AT1-receptor had a higher circulating endothelial microparticles leve than theautoantibodies against AT1-receptor negative group. And EMP did not differ betweenautoantibodies against alpha1-adrenergic receptor positive group and autoantibodiesagainst alpha1-adrenergic receptor negative group. Autoantibodies againstAT1-receptor may play a role in the produce of EMP by means of mediating vascularendothelial damage.