| Objective:SPA-1 and PD-1 have been reported as regulators involved in autoimmune diseases. To explore their roles in pathogenesis of SLE and their relationships to clinical parameters, expressions of SPA-1 and PD-1 in both skin lesions of patients with SLE and renal tissue of lupus nephritis with different types were systematically investigated.Methods:Renal biopsy and skin lesion samples and control groups were collected from the union hospital during 2010.1-2010.7. All patients'clinical parameters were collected and well recorded. The expression of SPA-1 and PD-1 was detected by immunohistochemistry method with monoclone anti-human SPA-1 antibody (gifted from Dr. Minato Nagahiro, Japan) and anti-PD-1 antibody (got from Co.Bios,Beijing, cat No. bs-1867R ). Semiquantitative expression analysis was performed by Image-Pro Plus 6.0. The relationship between SPA-1 and/or PD-1 and clinical parameters was analyzed respectively by ANOVA analysis. P<0.05 was considered significant.Results:1. SPA-1 was first found high expression in spinous layer and basal layer of epidermis in skin lesions of SLE, but was only expreesed in basal layer of epidermis in controls groups; PD-1 wasn't found its expression in skin lesions of both SLE and control groups. 2. SPA-1 and PD-1 were mainly expressed in renal tubules in both lupus nephritis and controls. But the expression levels of SPA-1 and PD-1in kidney of lupus nepritis were significantly higher than that in controls. Expression levels of SPA-1 were higher in renal tissue of typeâ…¤lupus nephritis than that of typeâ…£+â…¤lupus nephritis. SPA-1 expression levels were the least in typeâ…£lupus nephritis, while expression levels of PD-1 were opposite to that of SPA-1 in renal tissues from lupus nehritis.3. Further analysis showed that in renal tissue of lupus nephritis expression level of SPA-1 was found negative correlation with that of PD-1 (R=-0.690, P <0.01).4. Expression levels of SPA-1 in renal tissue of lupus nepritis were negatively related to deposition quantity of IgM (R=-0.335), C3 (R=-0.522), C1q (R=-0.712) in renal glomeruli, renal interstitial demage index (R=-0.372), chronic nephritis activity index (R=-0.364); Expression levels of PD-1 in renal of lupus nepritis were positively related to acute nephritis activity index (R=0.395), deposition quantity of IgM (R=0.373), C3 (R=0.472), C1q (R=0.615) in renal glomeruli,renal interstitial demage index (R=0.537).5. Expression levels of SPA-1 in renal tissue of lupus nepritis were negatively related to IgA (R=-0.399), antibody titers of anti-dsDNA (R=-0.751), anti-histone (R=-0.667), anti-nuclosome (R=-0.936), anti-ribosomalP (R=-0.936) in sera, positively related to IgM (R=0.34), C3 (R=0.833)/C4 (R=0.674) in sera.6. Expression level of PD-1 in renal tissue of lupus nepritis was negatively related to C3 (R=-0.565)/C4 (R=-0.496) in sera, positively related to antibody titers of anti-dsDNA (R=0.632), anti-histone (R=0.753), anti-nuclosome (R=0.561).7. Expression level of SPA-1 in renal tissue of lupus nepritis was positively related to the number of blood platelets (R=0.680) and leucocytes (R=0.765), negatively related to proteinuria (R=-0.509), hematuria (R=-0.836), number of leucocyte in urine, urine protein/24h (R=-0.702), SLEDI (R=-0.36). Expression level of PD-1 in renal tissue of lupus nepritis was positively related to SLEDAI (R=0.495), number of leucocyte in urine (R=0.907), but negatively related to number of leucocytes (R=-0.35), number of erythrocytes (R=-0.878) in blood.Conclusion:SPA-1 and PD-1 was over expression in renal tissue of SLE, and related to many clinical parameters. These dates suggest that the abnormal expression of SPA-1 and PD-1 may play important roles in pathogenesis of SLE. |
PDF Full Text Request