| Chelidonine is a benzophenanthrene alkaloid as main active constitutented in traditional Chinese drug chelidonii (exsiccation of Chelidonium majus L.). Sufferers of cancer are more or less suffering varying degrees of pain during the disease process. If the pian will not to be treated promptly, it will induce a series of serious disord like functional disorder of nerve, endocrine disturbance, hypoimmunity, etc. This will not be a good for treating on cancer. Chelidonine is used to relieve pain on clinic for a long time, it used to relieve the pain caused by the chemotherapy or non-chemotherapy to lung cancer, cancer of the liver, gastric cancer and so on. The research on pharmacologic action of Chelidonine show that Chelidonine has anti-tumor function, which make people pay close attention to it. The forthcoming research in domestic and foreign show that Chelidonine has a conclusive anti-tumor function, but the molecular mechanisms on anti-tumor function will be studied deeply and systematic.Cancer is a kind of cell-cycle disorders. Studies of drugs on tumor cells in cell cycle regulation to find a new target for treatment of cancer through this way caused by people's close attention. Mitotic phase(M phase) is the most important ingredient in cell cycle, it has a complicated and refined adjusting process. A great quantity of regulatory protein participate in mitosis like circulation of centrosome, spindle checkpoint, microtubule- centromere glutinous, spindle pack, chromatin condensation, etc. Error happen in this process will induce chromosome labilization, aneuploid emerged and tumor emerged. This article is research on regulation of cell cycle related genes and p-H3 Ser10 proteinum expression after Chelidonine treated. Perspective of anti-cancer molecular mechanisms for further development of anticancer drugs provides the scientific basis.MTT assay and SRB assay was used to evaluate the effect of Chelidonine on the rates of cell proliferation in this issue. The results showed that after treated for 48h, Chelidonine can inhibit the grows of SGC-7901 cells significantly and showed a dose-dependent, IC50 are 24.55μmol/L and 23.41μmol/L.Then cellcycle of SGC-7901 cells treated by Chelidonine were analyzed by FCM and it showed that after treated by Chelidonine of 6.25μmol/L,12.5μmol/L, 25μmol/L for 48h, percentage of G2/M stage of SGC-7901 groups was increased with the increase of drug concentration.. At last we used wetern blotting to detecte the influence of Chelidonine on G2/M phase-associated protein expression of cdkl,p-cdk1(Thr14), cyclinB1, and p-H3 SerlO in SGC-7901 cells. The results showed that after treated by Chelidonine of 6.25μmol/L,12.5μmol/L,25μmol/L for 48h, the expression of cdk1, cyclinB1 and p-H3 Ser10 protein were down-regulated (P<0.05 or P<0.01) and that of p-cdk1(Thr14) was upregulated(P<0.05 or P<0.01), compared with the control group, all of the datas have statistically sgni-ficant differences.Summarily, Chelidonine can induce arrest of G2/M phase in SGC-7901 cells through reducing complex formation of cdk1-cyclinB1, meanwhile up-regulating the expression of p-cdkl(Thr14) to inhibit the activity of cdkl or complex formation of cdkl-cyclinBl. Chelidonine can reducing the phosphorylation of H3 Ser10, show Chelidonine can induce arrest of M phase in SGC-7901 cells, the molecular mechanisms expected to deeply study. |
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