Study On Repair Of Adult Rat Peripheral Nerve Injury By Transplants Of Olfactory Ensheathing Cells

ObjectiveThe objective of this study is to explore the culture methods of separation and purification of the adult rat olfactory ensheathing cells (OECs) and to explore the active effect on regeneration of peripheral nerve after injury by Transplants of Olfactory Ensheathing Cells.MethodsAfter deriving, the purified culture methods of differential adherence combined with cytarabine (arabinosyl cytosine) inhibition is used to culture olfactory ensheathing cell. The process is observed at different stages and at the same time conduct immunohistochemical staining of glial fiber acidic protein (GFAP) and nerve growth factor receptor p75 (NGFRp75).30 Wister rats were randomly divided into control saline group (negative control group), olfactory ensheathing cells (OECs) group, a cobalt amine group (positive control group) respectively. Clip the right side of the rat sciatic nerve to produce injury model and inject nerve drugs with micro-syringe injections. Saline group receives normal saline 10ul; olfactory ensheathing cells are injected 10ul cell suspension; methylcobalamin is injected mecobalamin 10ul (0.5mg/ml). After complete hemostasis, close incision layer by layer. Respectively, after one week, two weeks, there weeks, four weeks, observe sciatic nerve function index (SFI) and kill the rats at the 4th week, take triceps to weigh wet weight and measure recovery rate of the triceps' wet weight.ResultsUse improved Nash differential adhesion to culture olfactory ensheathing cells. One day later, add Ara-c5μmol/L and make it function for 24-48 hours to develop olfactory ensheathing cells with sufficient number and high purity. After operation, each group, at one week, two weeks, there weeks, four weeks respectively, shows restoration of the sciatic nerve, but the olfactory ensheathing cells (OECs) group and cobalt mecobalamin group are better than saline saline group and significant difference is significant (P<0.01). Olfactory ensheathing cells (OECs) group and methylcobalamin group has no significant difference. Four weeks after opration, weigh triceps muscle'wet weight and compare the amount of damage with the contralateral. Methylcobalamin group is 0.7628±0.4295; olfactory ensheathing cell group is 0.7652±0.5630; saline group is 0.7052±0.0525. The difference between the former two anf the latter is significant (P<0.01) and there is no significant difference between the former two.ConclusionThe purified culture method of differential adherence combined with cytarabine (arabinosyl cytosine) inhibition is a simple, practical and economical method to culture olfactory ensheathing cell. And culturing Olfactory Ensheathing Cells has an positive effect on the recovery of peripheral nerve after injury.