A Preliminary Study On Differentiation Of Adipose-derived Stem Cell To Odontoblast-like Cell

[Objectives] A preliminary study on differentiation of adipose-derived stem cells(ADSCs) isolated from Sprague-Dawley rats into odontoblast-like cells in the specif conditioned medium[Methods]①The adipose tissue was gained from the groins of the four Sprague-Dawley neonate rats born before there days, the cells were got by digestion. The expression of the second passage cells surface antigen CD44,CD105 by streptavidin peroxidase method.②To observe the growth of the second passage cells by MTT.③The second passage cells were isolated and cultured, transforming growth factorβ1(TGF-β1) (2μg/mL), dentin noncol-lagenous proteins(DMNCP) (200μg/mL),TGF-β1(2μg/mL)+DMNCP (200μg/mL) were supplemented in culturemedium., the blank group was established. RT-PCR was also used to identify the expression of DSPP.[Results]①The the second passage cells expressed CD44,CD105 by streptavidin peroxidase method.②The the second passage cells quickly growrd in 3-5 days by MTT.③The the second passage cells were cultured for 6 days in culturemedium,RT-PCR showed the cells in these two groups in DMNCP and TGF-β1+DMNCP groups expressed specific dentin sialophosphoprotein (DSPP), these other two groups in blank and TGF-β1 groups did not expressed DSPP. [conclusions] ADSCs of the Sprague-Dawley neonate rats could be induced to odontoblast-like cells in culturemedium supplemented DMNCP and TGF-β1+DMNCP, DMNCP acts as the key effect in this process.this is favourable experiment foundation for the differentiation ADSCs to odontoblast cells.