| Objective: Bone marrow mesenchymal stem cells (BMSC) are key components of the hematopoietic microenvironment. Both hematopoietic cells (HC) and BMSC can probably be altered in response to similar damage-inducing factors, and cells have similar genetic predispositions. Our study indicates whether they have cytogenetic aberration and provides evidence of their important role in pathogenesis of MDS.Method: We establish the culture system of BMSC from MDS patients and work on their phenotypic(morphology and immunophenotype) properties. We performed standard cytogenetic analyses of both HC and BMSC from 22 patients with myelodysplastic syndrome 7 healthy individuals. Mononuclear cells were isolated from fresh bone marrow aspirates at the time of initial diagnosis for cytogenetic analysis of HC and we adherent culture and identify the BMSC successfully.Result: In terms of morphology, as well as the expression of certain cell markers, no differences are observed between BMSC from MDS patients and those deriverd from normal marrow. In both cases, BMSCs express CD29, CD90, CD73, in contrast,they did not express CD45, CD34. Cytogenetic analyses of BMSC are successfully performed in 22 cases. Cytogenetic aberrations are observed in BMSC from 14 (64%) MDS patients, usually involving the loss of chromosomal material. We observe a random loss of chromosomal material in significant proportions of BMSC. A high proportion of random loss may be a marker of chromosomal instability of BMSC from MDS patients.Conclusion: BMSC from MDS patients show chromosomal abnormalities. Our data probably reflect functional BMSC alterations in MDS patients. Although the majority of cytogenetic aberrations in BMSC were not clonal and differed from chromosomal markers in HC from the same individual, detection of high proportion of random loss in BMSC suggests enhanced genetic susceptibility of these cells in MDS. This may indicate potential involvement of BMSC in the pathophysiology of MDS. |
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