The Study Of Structure And Bioactivity Of The Polysaccharide Extracted From The Mycelium Of Paecilomyces Hepiali

The polysaccharide of CPHP was isolated from Paecilomyces hepialis by hot water.The water-extracted polysaccharide CPHP was fractionated by frozen-thawed, deproteinated by Sevag method, and purified by anion-exchange chromatography on a column of DEAE-Sepharose CL-6B and gel filtration chromatography on a column of Sepharose CL-6B. Then fraction were obtained and named as PHP.HPLC analysis showed that the polysaccharide of PHP was homogeneous components, and the average molecular weights was 28kDa.GC analysis showed that PHP was composed of Man,Gla,Glc,and small content of Rha. The molar ratio was 13.36:8.18:3.59:1. The partial acid hydrolysis of PHP showed that the main chain of PHP was composed of Man and Gal, The end of sugar chain or branched-chain was composed of Glc,Gal and Rha. Periodate oxidation and Smith degradation analysis suggested that PHP was mainly contained 1â†',1â†'6 bond type.In addition, there were 1â†',1â†'2,1â†'2,6,1â†'4,1â†'4,6 in PHP.The methylation analysis applied for PHP show that there were six bond type in PHP.Man was detected in 1â†'2 and 1â†'6;Gal was detected in 1â†'and 1â†'2,6;Glc was detected in 1â†'6; Rha was detected in 1â†'2.All of them could be Oxidized by Periodate. The molar ratio of Man,Gal,Glc and Rha was 2.90:1.73:0.69:0.19.It was are in agreement with GC analysis.CPHP was cultivated with lymphocyte cells.Between 100μg/ml to 400μg/ml, CPHP could induce lymphoctes proliferation,and the higher the concentration, the effect is more obvious. At the dose of 100 and 200μg/ml, CPHP can promote the ConA-induced proliferation of T lymphocytes, However, it did not affect the LPS-induced proliferation of B lymphocytes.At the dose of 50,100,and 200 mg/kg,CPHP could inhibit tumor growth in S180 tumor-bearing mice .tumor inhibition rates were 0.32±0.15,0.44±0.18,and 0.34±0.24.they are lower than the positive control group of Cyclophosphamide.meanwhile, Spleen index of CPHP treated group was significantly higher than the negative control group at the dose of 100mg/kg.however, Among the dose of 50,100,and 200 mg/kg,thymus index of CPHP treated group was lower than the negative control group.ELISA analysis of TNF-αshowed that CPHP could increase the expression of tumor necrosis factor-alpha (TNF-α) in serum at the dose of 100mg/kg and 200mg/kg,and the higher the concentration, the effect is more obvious. These results indicated that the tumor inhibitory activity of the CPHP may be related to the stimulation of immune system, which has a great significance in therapeutics of the tumor growth.