The Comparative Study To Acute Spinal Cord Injury Between Intravenous MP And Epidural Injection Of MP

BackgroundAcute spinal cord injury is the kind of serious nervous system damage, easily lead to sensory function below the injury level and the partial or total loss of motor function, the field of spinal surgery is a common and devastating disease.Treatment with methylprednisolone spinal cord injury have been numerous reports at home and abroad, with spinal cord injury physiology, biochemistry, pathology and clinical research continues to deepen, spinal cord injury has also been rapid development. In recent years, different scholars using different methods in spinal cord injury patients, have made some effect, also reported some of the drugs Contribute to functional recovery after spinal cord injury, but the results are still unsatisfactory, in order to seek a new method of treatment of spinal cord injury, according to the pathophysiological changes after spinal cord injury, athophysiologic changes to minimize this will be the future of a effective treatment. Systemic application of drugs and drug through the blood circulation channels directly to the spinal cord injury site, may be effective to interrupt the pathophysiological processes after spinal cord injury or mitigated to achieve the treatment of spinal cord injury, spinal cord function of protection or restoration purposes. Therefore, further research a better variety of treatment programs and drug use could be a very promising approach.Objective(1)Application of Intravenous of methylprednisolone, intrathecal injection of methylprednisolone in the treatment of spinal cord injury and their behavior changes;(2)Application of Intravenous of methylprednisolone, intrathecal injection of methylprednisolone and the combination of the two pathological changes in spinal cord injury;(3)Application of Intravenous of methylprednisolone, intrathecal injection of methylprednisolone and the combination of the two treatment effects on the injured spinal cord blood supply;(4)The results of different drugs through the program on acute spinal cord injury in rats, the clinical application of drugs for the treatment of spinal cord injury to provide theoretical guidance.Materials and MethodsExperiments will be 64 and the control group rats were divided into 3 different treatment drug group. Carried out by intraperitoneal injection of anesthetic drugs, the use of improved Allne's fight against acute spinal cord injury rat model. 5 minutes after surgery, respectively, via the tail vein Intravenous methylprednisolone, intrathecal injection of methylprednisolone and the two combined to give medication. After 7 days in detecting the following indicators: (1)hurdle rates and BBB score obstacles Rvilin ramp rate behavior of rats with spinal cord injury evaluation; (2)behavioral score after the rats were killed and produce pathology, the normal HE staining Slice; (3)ECT scan through the local blood flow compared the number of damaged sites, and statistical analysis.Results(1)With prolonged injury, ramp angle of each group were increased to different degrees and varying degrees of lower hurdle rates, but at different time points compared with the combined treatment group the control group, intravenous methylprednisolone group, intrathecal injection of methylprednisoloneLong the difference between the groups: combination group, intravenous methylprednisolone group, intrathecal injection of methylprednisolone group at different time points on the ramp angle is lower than the control group (P<0.05), significantly higher than the hurdle ratesthe control group (P<0.05), combined treatment group at different time points higher than the ramp angle of intravenous methylprednisolone group, intrathecal injection of methylprednisolone group (P<0.05), barriers were significantly lower than intravenous methylprednisoloneLong group, intrathecal methylprednisolone group (P<0.05), intravenous methylprednisolone group at different time points higher than the ramp angle of intrathecal methylprednisolone group (P<0.05), significantly lower than the hurdle ratesintrathecal injection of methylprednisolone group (P<0.05).(2)Pathological changes: treatment group discontinuity after spinal cord injury, local swelling significantly, was subdural hemorrhage, white matter, gray matter blurring, light microscopy: local bleeding damage to the spinal cord, nucleus uptake, cell necrosis and dissolution of neurological and inflammatory cell infiltration, intravenous methylprednisolone group, compared with combined spinal cord injury in intrathecal methylprednisolone group was significantly lighter.(3)Damaged part of the spinal cord blood flow change: the combination group and control group injured spinal cord blood flow was no significant difference (P>0.05), intravenous methylprednisolone group, intrathecal injection of methylprednisolone group of damaged spinal cord blood No significant difference in flow (P>0.05), combined treatment group and blank control group and the intravenous methylprednisolone group, intrathecal injection of methylprednisolone group of injured spinal cord blood flow was significantly different (P<0.01).Conclusions(1)Intravenous methylprednisolone group, intrathecal injection of methylprednisolone group and combined group on the treatment of spinal cord injury in rats with spinal cord injury can behavioral improvement than the control group;(2)Intravenous methylprednisolone group, intrathecal injection of methylprednisolone group, the combination of group therapy can reduce the pathological changes of spinal cord injury;(3)Combined group on spinal cord injury can damage parts of the spinal cord blood flow compared with intravenous methylprednisolone group, intrathecal injection of methylprednisolone group increased significantly, the combination of group and no significant difference between the control group.