Fluorometric Analysis Of Active Components Of Chinese Medicine Common Camptotheca Fruit, Fructus Aurantii Immaturus And Establishment Of 3D Fluorescence Fingerprint Of Fructus Arctii

Fluorescence spectra of camptothecin and 10-hydroxycamptothecin in Chinese medicine Common Camptotheca Fruit (Xishuguo in Chinese) and synephrine in Fructus Aurantii Immaturus (Xinfulin in Chinese) were studied in this thesis. Influences of experimental conditions on fluorescence spectra were also investigated.? A solution fluorometric method were established for the analysis of camptothecin in CCF, and a thin-layer fluorescence scanning method were established for the analysis of camptothecin and 10-hydroxycamptothecin in CCF and synephrine in FAI. Three-dimensional fluorescence spectrum of Fructus Arctii (Niubangzi in Chinese) and was studied, and a simple, reliable method for quality control of Niubangzi was established.Three parts were included in this thesis:1. Fluorescence spectra of camptothecin and 10-hydroxycamptothecin, two active components of CCF were studied.? It was found that acidity had great effect on the fluorescence of camptothecin and 10-hydroxycamptothecin. Solvent and surfactant also had effect on fluorescence of 10-hydroxycamptothecin. Using L-tryptophan as a reference, fluorescence quantum yield of?camptothecin and 10-hydroxycamptothecin were measured to be 0.68 and 0.093, respectively. Fluorescence spectra of CCF methonal extraction were studied. A new solution fluorometric method and a?thin-layer fluorescence scanning method were established for the analysis of camptothecin in CCF.? Using the two methods, camptothecin in CCF sample were determined respectively to be 0.1273% and 0.1220%. Since the disturbance of camptothecin, 10-hydroxycamptothecin can not be analyzed by solution fluorometric method. A?thin-layer fluorescence scanning method were proposed for analysis of 10-hydroxycamptothecin in CCF. Using the method, 10-hydroxycamptothecin in CCF sample was determined to be 0.01274%.2. Fluorescence spectra of synephrine, an active component of FAI, were studied.?It was found that synephrine present strong and steady fluorescence in acidic and netural solutions. Using L-tryptophan as a reference, fluorescence quantum yield of? synephrine were measured to be 0.13. A? thin-layer fluorescence scanning method was established for the analysis of synephrine in FAI. Using the method, the content of synephrine in FAI sample was determined to be 0.3558%.3. 3D fluorescence spectra of Fructus Arctii and some drugs with similar fluorescence spectra were studied. The experimental procedure for obtaining 3D fluorescence spectra were investigated from aspects of specificity, reproducibility and robustness of the method,arctiin in Fructus Arctii was determined to be 6.58%. 3D fluorescence fingerprint of Fructus Arctii was established.