Effects Of Acute Phase Serum Of The Children With Kawasaki Disease On The Human Umbilical Vein Endothelial Cells Function

Objective To observe the effects on proliferation, migration and production of nitric oxide (NO) in human umbilical vein endothelial cells (HUVEC) cultured with the serum of the children with Kawasaki disease in acute phase, in order to further understand the possible mechanism of vascular endothelial injury with Kawasaki disease.Methods HUVEC were cultured in vitro, and the serum of the normal children and the children with Kawasaki disease were prepared, then HUVEC were cultured with the above serum. The proliferation, the NO production, and the migration of HUVEC were detected by MTT test, NO detection kit, and the transwell chamber methods, respectively.Results The OD value of the HUVEC detected by MTT test after being cultured with the serum of the children with Kawasaki disease in acute phase(0.318±0.033) was significantly lower than those of the control group and the normal children (0.700±0.109,0.565±0.071) (P<0.01). The NO produced by HUVEC which were cultured with the serum of the children with Kawasaki disease in acute phase (5.618±1.149μmol/L)were significantly lower than those of the control group and the normal children (8.779±2.059,12.138±0.478μmol/L) (P<0.05).The numbers of the HUVEC through the aperture 8μm polycarbonate membrane cultured with the serum from the children with Kawasaki disease in acute phase (29.500±6.364)were significantly less than the normal children(50.000±1.414)(P<0.05), but no significant differences were ocurred compared with the control group(14.000±4.243).Conclusion The serum of Kawasaki disease in the acute phase could inhibit the proliferation of HUVEC, reduce the NO production of HUVEC, inhibit the migration of HUVEC.