Whole Exome Capture Sequencing Identifies Candidate Genes Related To HCC

Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and exhibits an inherited predisposition to infiltrate and metastasize. Here, we use exomic sequencing parallel sequences on an Illumina Solexa platform to identify the somatic mutations in ten HCC patients with portal vein tumor thrombus (PVTT), the intrahepatic metastasis. Through sequencing 29 samples of ten HCC patients'non-cancerous livers, primary and PVTT (one of PVTTwas degradation) we found 122574 somatic mutations on ten HCC patients with PVTT.After analyzed according to described method, we selected 381 non-silenced mutations which located on exon for futher test by sanger sequencing. Finally we retested 165 non-silenced mutations, validation rate was 43%. Base transitions and base transversions were frequently found among 165 confirmed non-silenced mutations. We assessed the role of 91 mutated genes in cellular survival via RNA interference; the results suggest that TMEM35, CDK14, HOXA1, ABCA1 could be potential cancer genes that confer growth and infiltration capabilities to HCC cells. For futher study we found TMEM35 gene, which encode a tetraspanin, have an important role in cell growth of hepatoma cells.