| Evodia is Rutaceae plants as Euodia rutoecarpa (Juss.) Benth., E. rutaecarpa (Juss.) Benth. Var. Officinal is (Dode) Huang or E. rutaecarpa (Juss.) Benth. Var. bodinieri (Dode) Huang dry nearly ripe fruit. Evodia first contained in the "Shen Nong' s Herbal Classic", is famous authentic ingredients in Guizhou Province. Evodia Weixinxingre, with a role of "Wentongxinyang" cmmonly used in clinical as cold medicine. For thousands of years, Chinese medicine believe Xinre drugs have significant efficacy, more vigorous, and go through the five internal organs, heart, liver, kidney, mainly as medicinal characteristics.This experiment studied in detail the results of previous studies based on comparative advantages and disadvantages of each method and the existing experimental conditions, Guizhou Yuqing GAP planting base Evodia was used as representative, in line with traditional, simple, principle and easy to reproduce on a set of basic research.1,Orthogonal optimization of Evodia herbs extraction.orthogonal experimental design were Optimized in Evodia water extraction, alcohol extraction conditions, research showed that:water extract of Evodia optimum conditions:with 10 times the volume of water, boil times, each time 120 minutes, water extract of Evodia optimum conditions:60% with 6 times the amount of ethanol extracted three times, each time 120 minutes. by comparing HPLC determination contrast, TLC pattern method, HPLC feature print, on the water extract, alcohol extract of Evodia drying methods of investigation, the results showed that: vacuum drying, microwave drying, freeze drying of extracts quality have no significant impact, so we can choose the appropriate drying method in think of energy efficiency, reduce consumption, saving time and other aspects according to the actual situation. 2,HPLC Feature print research of Evodia water and alcohol extract.Evodia water, alcohol extract feature print by HPLC were reached by system, established a feature print Evodia Extract Method:The column was ZORBAX Eclipse XDB-C18 (4.6×150 mm,5μm), mobile phase of acetonitrile -0.1% formic acid water, use of the mobile phase gradient elution system (time:0 min～5 min～38 min～65min, the proportion of acetonitrile:5%～5%～23%～100%), the flow rate was 1mL·min-1, detection wavelength of 258nm, column temperature was 30℃. Feature print analysis by the study methodology, precision, stability, reproducibility are good, while ethanol extract of Evodia marked the peak and 20 water extracts of 19 peaks.3, Qualitative research of Evodia extract feature print spectra peaks.By use ESI-MS builded a UPLC-MS/MS analysis method, based on the literature, reference compounds contained in Evodia chromatographic behavior and mass of the pyrolysis law, and by optimizing the gradient elution Mass of the performance parameters,8 of active ingredient were identified the, there are chlorogenic acid and its isomer, quercetin-3-0-β-D-galactosidase, dehydrogenase evodiamine, limonin, 14-formyl-dihydro rutaecarpine, evodiamine and rutaecarpine. LC-MS can rapidly and accurately identify Evodia extract the main active ingredients, enriching the feature print chemical information reflected, also to better clarify the role of the material basis of traditional Chinese medicine and provide a basis for the mechanism.4,Chemical constituents and biological activities compare of Evodia water and alcohol extract, Comparison of "Wentongxinyang," drugs feature print.Evodia water, alcohol extract of the transfer of alkaloids were 5.45% and 71.22%; In addition, in comparing of Evodia water, ethanol extract and herbs HPLC Feature print we found that water, alcohol extract similarity to herbs were 0.933 percent and 0.545 percent, two results both showed that ethanol extract of the chemical composition of reserves for medicinal extract better than water. Through comparison HPLC Feature print of the water extract, ethanol extract, we found that extracts of different polarity, there is difference between the peak area. There are three "Wentongxinyang" drugs (Evodia, aconite, ginger) HPLC Patterns of water extracts we found no peaks in common, initially prove that they have no common substances. Efficacy of screening by further experiments showed that ethanol extract of the rat left ventricular pressure and heart rate greater than the promotion of the role of water extract and ethanol extract confirmed the quality is better than water extracts, this result is effectively Evodia part of the basis for further positioning, also provide a strong clinical drug using reference. |
PDF Full Text Request