The Research On The Toxic Effect Of The Multiplex Biological Preparation Against The 4th Larva Of Culex Pipiens Pallens Coquillett

Culex pipiens pallens Coquillett can bring harm to human health by transmitting Bancroft's filariasis and epidemic encephalitis B, so it harms human health and belongs to the dominant mosquito of rural-urban living area in north China. Furthermore there is a vital question of the pest control which Culex pipiens pallens Coquillett easily becomes resistant to some often-used insecticides. The macrofungus and insecticidal activity plants are selected in this article,and to make the macrofungi liquid fermentation productions and the ethanol extractss from plant into the hybrid biological mosquito-killed preparation, the results are follows:1,This article screens out Amanita pantherina (Dc.:Fr.)Schrmm and Amanita citrina (Schaeff.) Pers. ex S., which the LC50 of the extracts from the macrofungi liquid productions to tested larva respectively are 58.945mg/mL and 59.726mg/mL;anothers are Artemisia capillaris Thunb and Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag., which the LC50 of the ethanol extracts from plant to tested larva respectively are 0.039mg/mL and 0.026mg/mL by doing the insecticidal activity trial on 52 kinds of tested macrofungus which belong to 18 families and 32 kinds of tested plant which belong to 14 families with standard as the toxins efficiency reaches 90% or over 90%.2,Mix the 2 kinds of extracts from the macrofungi liquid fermentation productions and 2 kinds of ethanol extracts from plant respectively according to 1000:1 as the mass ratio, and then get 4 kinds of different combinations, test their LC50. The LC50 of this combination whose constitution is the extracts from Amanita pantherina (Dc.:Fr.)Schrmm liquid fermentation productions with the ethanol extracts from Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. is 27.91mg/mL and its cotoxicity coefficient is 64.71 by calculation, thus its toxicity is the strongest. So this combination is selected as the original compound of the multiplex biological mosquito-killed preparation.3,Machining the original compounds to make that become a right formulation by the"method of ultrasonic phase inversion", then get the multiplex biological preparation- micro-emulsion, and it accords with a series of correlative quality index. Formula: 20.02% original compound, that is Amanita pantherina (Dc.:Fr.)Schrmm liquid fermentation production: the ethanol extract from Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag.=1000:1;0.14% emulsion: emulsifier ABSCa: agricultural emulsifier No.600=5:7; 0.01% coemulsifier: isopentanol;0.025% dispersant: SDS: sodium lignosulphonate=1:1; 7% antifreeze: glycerin: NaCl saturated solution=7:3; 0.1% defoamer: 30% silicone defoamer T-210; 0.1% antimicrobial: potassium sorbate; water: the tap water in antichloration. Add the aqueous phase with a speed that less than the critical speed before the phase inversion as far as the cubage of aqueous phase is greater than 74% bulk volume, and that can add the aqueous phase with any speed as far as achieve 100% bulk volume until finishing the phase inversion. This pilot is machining 50mL system, the critical speed is 0.5m/s. The LC50 of micro-emulsion against the 4th larva of Culex pipiens pallens Coquillett is 139g/L, and the 96hLC50 against the Danio rerio is 4520mg/L, so is graded as lower toxicity.4,The mechanism of action of this preparation on the 4th larva of Culex pipiens pallens Coquillett includes: contact toxicity and fumigation action.5,This micro-emulsion should be in a opaque and well hermetical plastic bottle, and store it in a cool and dark area on the understanding that the temperature range of -2℃~28℃, also try to avoid producing foam in transport. When in use, the local concentration shall not be lower than 139g/L, and not good using it in rainy day or high direct sunlight. Don't allow this micro-emulsion come into direct contact with eye, nasal cavity, oral cavity,respiratory tract, and cutis, rinsing with eau doue if touch it carelessly, and immediately hospitalize if necessary.The innovations in the dissertation is using macrofungi productions on the Culex pipiens pallens Coquillett control, and with the ethanol extracts from plants to make it into a hybrid biological toxin mosquito-killed preparation. By the ultrasonic, the high-intensity shearing force is supplied, so enhancing the emulsifying effect with the "method of ultrasonic phase inversion", and then the original compounds into the more novelty micro-emulsion at home and abroad currently.