Mesenchymal Stem Cells Were Researched In Allogeneic Hematopoietic Stem Cell Transplantation Of Children

Part: Umbilical cord mesenchymal stem cells in culture,indentification and ultrastructureObjective: The purpose of this study was to isolate and culture mesenchymal stem cells(MSCs) from full-term newborn umbilical cord and to observe the ultrastructure of humanumbilical cord mesenchymal stem cells (hUCMSCs).Methods: UCMSCs were isolated and cultured by collagenase digestion, and thensubcultured, amplificated, and observed cell morphology under the microscope. Weanalysed the immunophenotype and trilineage differentiation potential of the third period ofhUCMSCs.Transmission electron microscopy and scanning electron microscope were used to observethe ultrastructure of hUCMSCs.Results: The appearance of hUCMSCs is spindle-shaped and polygonal, and nucleuswere observed; hUCMSCs express CD44, CD73, CD105, not CD34, CD45, CD31andhuman leukocyte antigen HLA-DR; hUCMSCs have the capacity of adipogenic,osteogenic,cartilage differentiation; short and thick microvilli processes can be seen at thesurface of hUCMSCs under scanning electron microscope; hUCMSCs can be seen twodifferent cell morphology under transmission electron microscopy. The one is in aquiescent period, which can be seen a large and round or oval nucleus with only onenucleolus, cytoplasmic organelles were less; the other is in a relatively active period, thesame cells can be seen one or two nuclei, the number of organelles is rich, structure is clear,can be seen visible expansion of the mitochondria.Conclusion: The cells which were successfully isolated and cultured from theumbilical cord have the biological characteristics of MSCs, and have two different states ofthe ultrastructure. PartⅡ::Observe curative effect of human umbilical cord mesenchymalstem cells for treatment of acute graft-versus-host disease of childrenObjective: The purpose of this study was to observe curative effect and security ofhuman umbilical cord mesenchymal stem cells (hUCMSCs) cure acute graft-versus-hostdisease of children.Methods: HUCMSCs were isolated and cultured by collagenase digestion, and thensub cultured. We use hUCMSCs with the third period to the fifth period for clinicaltreatment. Five cases of children with acute leukemia achieved complete remission afterchemotherapy.Two cases received bone marrow hematopoietic stem cell transplantation withHLA3/6matched. One case received hematopoietic stem cell transplantation of bonemarrow and peripheral blood from an HLA-matched family donor. One case receivedumbilical cord blood hematopoietic stem cells transplantation of unrelated donor withHLA4/6matched. One case received umbilical cord blood hematopoietic stem cellstransplantation of unrelated donor with HLA5/6matched. The children receiveimmunosuppressive therapy after occurring Ⅲ-Ⅳ degree acute graft-versus-host disease(aGVHD). They receive hUCMSCs (0.5×106/kg, body weight) infusion when appealtherapy is invalid.Results: The Five cases of children with acute leukemia achieved hematopoieticreconstitution and developed the Ⅲ-Ⅳ degree aGVHD of the skin, the liver and thegastrointestinal tract. The immunosuppressive therapy was invalid. The five cases of childrenwere infused hUCMSCs. The rash subsided, the liver function returned normal and thegastrointestinal symptoms improved. They did not develop infusion-related adversereaction. At present, the five cases of children were not recurrence of primary disease andwere in a state of disease-free survival.Conclusion: Allogeneic hematopoietic stem cells transplantation is an effectivetreatment method in children with acute leukemia. HUCMSCs can remedy aGVHD. It is safeand effective to infuse MSCs i.v. and no side-effects have been observed.